摘要利用渗透调节剂PEG-6000（以下统称为PEG）进行模拟干旱试验。通过研究5%，10%，15%,20%,25%等不同浓度胁迫下，大豆的生长情况与相关的渗透调节物质的变化，找出大豆的各个生理生化指标在干旱环境下改变。发现大豆的膜脂过氧化程度在不同浓度PEG胁迫下受到的影响，找到改变比较大指标,检测大豆的抗旱性能。结果显示：随着PEG浓度的增加，大豆的CAT活性呈现上升趋势，并且渗透调节物质:游离PRO、可溶性糖、可溶性蛋白以及MDA等含量都明显增加。此结果有助于大豆抗旱品种的鉴定与筛选。69171

毕业论文关键词：大豆  PEG-6000  干旱胁迫  生理特性  渗透调节物质

The effects of simutation drought with PEG on physiological characteristics of soybean

Abstract

Using osmotic adjusting agent PEG-6000 (hereinafter referred to as PEG) to simulate the drought stress experiment. The associated osmolyte of soybean was studied under the different concentrations of stress (5%, 10%, 15%, 20%, 25%, etc), to find the change of key physiological and biochemical indexes of soybean under drought environmental. Finding that soybean membrane lipid peroxidation in different concentrations of PEG stress affected and detecting soybean drought resistance. The results showed that: with the increase of PEG concentration, CAT activity of soybean hsve an up ward trend, and osmotic adjustment(ree PRO, soluble sugar, soluble protein and MDA, etc.) have increased significantly. The change of osmotic adjustment and peroxidation index under would help the identification and screening of drought-resistant varieties.

Key word: soybean  PEG-6000  drought stress  Physiological characteristics  Osmotic adjustment substance    

目录

摘要 1

关键词 1

Abstract 2

目录 3

图清单 4

绪论 4

1. 材料与方法 5

1.1. 实验材料 5

1.2. 实验设计 5

1.3. 测定方法 6

1.4. 数据分析 6

2 结果与分析 6

2.1  PEG模拟干旱对PRO含量的影响 7

2.2  PEG模拟干旱对可溶性糖含量的影响 7

2.3  PEG模拟干旱对可溶性蛋白含量的影响 8

2.4  PEG模拟干旱对CAT活性的影响 8

2.5  PEG模拟干旱对丙二醛含量的影响 9

3  结论与讨论 10

致 谢 10

参考文献 11

图清单

图序号 图名称 页码

图1 PEG模拟干旱对PRO含量的影响 7

图1 PEG模拟干旱对可溶性糖含量的影响 7

图3