牛耳朵幼苗对低温胁迫的生理响应
关键词： 苦苣苔科；室内花卉；栽培温度；生理指标
Physiological Response of Chirita eburnea Hance Seedling to Low Temperature Stress
Abstract：Gesneriaceae are a kind of new indoor ornamental flowers, but the gardening application was mostly developed by amateurs. And lack of systematic studies on the physiological and ecological, planting and breeding etc.. In this paper, we investigated on the response of Chirita eburnea Hance of Gesneriaceae Seedling to low temperature stress. Young plants were treated under 5 ºC for 12 h, 24 h, and 36h respectively, and then moved back to room temperature for 24 h, 48 h to observe the recovery. The physiological indexes such as chlorophyll fluorescence were measured. The results showed that, Fv/Fm decreased along with the increasing of chilling time and could be recovered to normal level after chilling. SOD activity and MDA content increased along with the treating duration and then could recover gradually. The relative conductivity increased with the increasing of low temperature treating time and could decrease during the recovery. Water content of leaves had a slight descending trend. The continuous 36 h 5 ºC low temperature treatment caused a certain physiological stress, but not critical damage, the young plants of Chirita eburnean can recover to normal growth after stress. In cultivation, it is preferred that we should ensure the winter ambient temperature above 5 ° C.
Key Words: Gesneriaceae;indoor plant; Cultivation temperature; Physiological Index
目 录
1 绪 论 1
1.1 苦苣苔科植物的分类和分布 1
1.1.1 苦苣苔科植物的形态特征 1
1.1.2 苦苣苔科植物的生态习性1
1.1.3 苦苣苔科植物的研究和应用情况 1
1.2 牛耳朵概述 2
1.2.1 牛耳朵的植物形态2
1.2.2 牛耳朵生态习性和应用 2
1.2.3 牛耳朵国内外研究现状3
1.2.4 低温胁迫的研究情况 3
1.3 牛耳朵的研究意义4
2 材料与方法 4
2.1 实验材料4
2.2 实验试剂4
2.3 实验器具和仪器设备 4
2.4 实验方法5
2.4.1 预备实验5
2.4.2 牛耳朵处理方法5
2.5 牛耳朵各项生理指标测定方法 5
2.5.1 PSII光化学效率（Fv/Fm）的测定5
2.5.2 叶片含水量的测定 6
2.5.3 电解质效率的测定6
2.5.4 SOD活性的测定6
2.5.5 MDA含量的测定6
3 结果与分析7
3.1 不同时间低温处理及恢复生长对光化学效率的影响7
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