摘要： 本课题从各种植物、花卉土壤中筛选能产β-苯乙醇的原核微生物菌株，并从菌种鉴定、产物鉴定以及工艺条件优化三方面开展研究。研究结果表明，结合16S rRNA以及生理生化实验，验证所筛选的菌株为肠杆菌科肠杆菌属的细菌。通过气相色谱法鉴定得出筛选的原核菌株可利用L-苯丙氨酸作为底物生物转化合成β-苯乙醇。在工艺优化方面，主要考察培养基碳氮比、pH值、培养温度以及培养转速对产量的影响。以β-苯乙醇产量为重要分析指标，结果显示在碳氮比为1:1，pH 7。0，转速150rpm，培养温度30-37℃条件下，β-苯乙醇产量较为可观，可稳定在0。6g/L左右。76158

毕业论文关键词： β-苯乙醇；原核菌株；艾式途径；生物转化优化 

Selecting, identification and characterization of prokaryotic microbial strains producing β-phenylethyl alcohol

Abstrcat: In this study, the prokaryotic microbial strains producing β-phenylethyl alcohol were screened from various plants and flower soils。 The study was carried out from three aspects: identification of bacteria, identification of products and optimization of process conditions。 The results showed that the strain was identified as bacterium of Enterobacteriaceae by 16S rRNA and physiological and biochemical experiments。 The purified prokaryotic strains were identified by gas chromatography to synthesize β-phenylethyl alcohol using L-phenylalanine as substrate for biotransformation。 In the process optimization, the effects of carbon and nitrogen ratio, pH value, culture temperature and culture speed on the yield were studied。 The results showed that the ratio of carbon to nitrogen was 1: 1, pH 7。0 , Rotation speed 150rpm, culture temperature 30-37 ℃ under the conditions, β-phenylethanol production is more impressive, can be stable at about 0。6g / L。

Keyword：β-phenylethyl alcohol；Prokaryotic strains；Ehrlich pathway；Biotransformation  Optimization

目录

1。绪论 - 1 -

1。1 β-苯乙醇的基本性质及应用 - 1 -

1。1。1  β-苯乙醇的基本性质 - 1 -

  1。1。2  β-苯乙醇的应用范围 - 1 -

1。2 β-苯乙醇国内外研究现状 - 2 -

1。3 β -苯乙醇的相关代谢途径 - 2 -

1。3。1  Ehrlich途径 - 2 -

1。3。2  β-苯乙醇从头合成途径 - 3 -

1。3。3  苯丙酮酸途径 - 3 -

1。4 微生物法合成β-苯乙醇 - 3 -

1。5本课题的研究目的和意义 - 4 -

2。材料与方法 - 6 -

2。1 材料与试剂 - 6 -

2。1。1 菌株及试剂 - 6 -

2。1。2 培养基 - 6 -

2。2 仪器与设备 - 6 -

2。3 菌株鉴定实验方法 - 7 -

2。3。1 革兰氏染色法 - 7 -

2。3。2 16s rRNA细菌序列测定 - 7 -

2。3。3 菌株生理生化鉴定 - 8 -

2。4 产物β-苯乙醇鉴定的实验方法 - 9 -

2。5 菌体生长曲线以及底物消耗曲线测定