新型银纳米阵列基板的SERS性能及农药啶虫脒检测研究
毕业论文关键字：表面增强拉曼光谱 农残检测 啶虫脒 表面改性
Study of the SERS Performance of Silver Nanorod Array and Its Application in Acetamiprid Detection
Abstract: The Surface-Enhanced Raman Scattering (SERS) technology with the characteristics of high sensitivity, low detection limit and rapid determination, is widely used in the field of biological detection, environmental monitoring, food safety and drug analysis. In this paper, surface modification is used for SERS active substrate, and the detection limit of acetamiprid is obtained, which provides a new method and technology for pesticide residues. Firstly, diluted nitric acid was used for removing the silver oxide from the surface of the silver nanorod array substrate. The probe molecular 1,2-bis(4-pyridyl)ethylene (BPE) was detected by Raman spectrometer at the wavelength of 785 nm, and obtained the SERS signal of substrate cleaned by different concentrations. The results show that the detection limit of BPE can reach 10-8 M at the nitric acid concentration of 4.914 M, and its sensitivity is 100 times higher than the one without surface modification. Next, the SERS signals of acetamiprid in different concentrations were detected, and the intensity changes with the concentration of acetamiprid solution. Simulation of acetamiprid vibration model by density functional theory determined the main characteristic peaks and the vibration mode of acetamiprid. The results show that the peak intensity of acetamiprid is increasing exponentially with the increase of the acetamiprid solution concentration, and the limit of detection is 1ppm.
Keywords: Surface-enhanced Raman spectroscopy Pesticide residues Acetamiprid Surface modification
目录
1 引言 . 1
1.1 农药的介绍与使用现状 . 1
1.2 农药的研究方法及研究意义 . 2
1.3 本论文的主要研究工作 . 3
2 表面增强拉曼光谱 . 4
2.1 表面增强拉曼效应的发现 . 4
2.2 表面增强拉曼效应的基本原理 . 4
2.3 表面增强拉曼基底的制备方法 . 5
2.4 利用倾斜角沉积法制备 SERS活性基底的应用及进展 . 7
3 银纳米阵列基板的性能研究 . 10
3.1 银纳米阵列基板 . 10
3.2 实验材料 . 10
3.3 实验仪器 . 11
3.4 实验模型 . 11
3.5 实验步骤 . 12
3.6 结果与讨论 . 14
3.7 本章小结 . 18
4 杀虫类农药啶虫脒的表面增强拉曼光谱研究 19