摘    要

目的：研究壳寡糖/化合物A纳米粒负载碱化阿霉素的可能性，评价制备所得的微粒纳米系统理化性质及其体外释放行为，为抗肿瘤药物治疗癌症提供一种新型药物载体。

方法:超声波分散法制备壳寡糖/化合物A空白纳米粒，芘荧光法测定壳寡糖/化合物A纳米粒临界聚集浓度，动态光分散法测定纳米微粒粒径和表面电位，透射电镜考查载药纳米粒的形态，并且研究其体外释放行为。

结果：实验室自制得到的壳寡糖/化合物A的实际接枝率为15。99%，空白纳米粒的临界聚集浓度为161。3ug/mL，空白纳米粒粒径和电位分别为506。7nm(PDI为0。239)和（63。5±4。99）mV，在碱化阿霉素理论投药量为50%时，壳寡糖/化合物A载药纳米粒的实际载药量为7。69%），包封率为81。39%，载药纳米粒粒径为327。9 nm (PDI 为0。320)，表面电位为(24。0±5。51)mV，体外释放结果表明药物的释放呈现PH敏感性。

结论：壳寡糖/化合物A纳米粒可以有效的包裹阿霉素成为粒径均一的纳米给药系统。该载药纳米粒具有PH敏感性以及缓释作用。壳寡糖/化合物A有望成为潜在的难溶性药物的载体材料，具有广阔的发展前景。85353

毕业论文关键词：壳寡糖/化合物A； 阿霉素； 纳米粒；体外释放

Objective: To study the possibility to prepare alkaline doxorubicin loaded chitosan oligosaccharide / compound A nanoparticles and to evaluate the physical and chemical properties and release behavior in vitro of the particulate drug delivery system, so as to provide a novel drug carrier for anti-tumor drugs。

Methods: Ultrasonic dispersion method was used to produce chitosan oligosaccharide / compound A blank nanoparticles; The pyrene fluorescence method was used to determine the CMC of chitosan oligosaccharide / compound A nanoparticles; Dynamic light scattering method was used to measure the particle size and surface potential; The morphology of drug loaded nanoparticles were examined by transmission electron microscopy to study the release behavior in vitro。源Q于W优H尔J论K文M网WwW.youeRw.com 原文+QQ75201.,8766

Results: The actual grafting rate of the lab made chitosan oligosaccharide / compound A nanoparticles was 15。99%。  The critical aggregation concentration of blank nanoparticles was 161。3ug/mL。 The nanoparticle size and Zeta potential of the blank nanoparticles were 06。7nm(PDI 0。239) and（63。5±4。99）mV respectively。 When the theoretical dose of alkaline doxorubicin was 50%, the actual grafting rate and encapsulation efficiency of chitosan oligosaccharide / compound A nanoparticles were 7。69% and 81。39% respectively。 The nanoparticle size and Zeta potential were 327。9 nm (PDI0。320) and(24。0±5。51)mV respectively。 In vitro release studies showed that the drug release was PH responsive。

Conclusion: Adriamycin can be effectively encapsulated into a uniform nanoparticle drug delivery system, namely chitosan oligosaccharide/compound A grafted nanoparticles, and the drug loaded nanoparticles had PH sensitivity and sustained release effect。 So it can be expected to become a potential carrier material for poorly soluble drugs, which has a broader development prospective。

Keyword: chitosan oligosaccharide / compound A ； doxorubicin； nanoparticles；in vitro release

目录

1前言 4

2实验器材 5

2。1材料 5

2。2仪器 5

3实验方法和结果 6

3。1壳寡糖/化合物A氨基取代度的测定 6

3。2空白纳米粒的制备