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摘要:目的：了解蚂蟥生殖周期性类固醇激素的相关变化规律。方法：运用酶解、萃取以及Uplc-Ms/Ms检测蚂蟥生殖周期中4种性类固醇激素。结果：蚂蟥雌三醇含量自4月龄逐渐上升，5月龄急速上升，6月龄达到最大值，7月龄急速下降,，8月龄又上升至极大值，之后逐渐下降；蚂蟥的雌酮的变化趋势基本和雌三醇变化趋势相同，5月龄开始明显的上升，在6月龄达到最大值，7月龄急速下降，8月龄又上升至极大值，之后逐渐下降；蚂蟥的睾丸酮3-6月龄缓慢上升，6-9月龄缓慢下降，9月龄含量达到最小值，之后极速上升，在11月龄达到了最大值；蚂蟥的黄体酮除了1、4、9、11四个月龄外均处于较大值，10月龄为最大值，11月龄快速下降至最小值。结论:6月龄的蚂蟥雌性激素含量达到最大值，10月龄蚂蟥孕激素含量达到最大值，10-11月龄的蚂蟥雄性激素急速上升达到最大值，蚂蟥性类固醇激素同步于性腺分化。29697
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毕业论文关键词：蚂蟥，性类固醇激素，生殖周期变化，性腺分化
A preliminary study on the changes of periodic steroid hormones in Whitmania pigra Whitman
Abstract: Objective: To investigate the changes of periodic steroid hormones in Whitmania pigra. Methods: Four kinds of steroid hormones were detected by enzymolysis, extraction and Uplc-Ms / Ms in the Whitmania pigra germination cycle. Results: The content of Whitmania pigra increased gradually from 4 months of age, and reached the maximum at 6 months. The 7-month-old decreased rapidly, and then increased to the maximum at 8 months, then decreased gradually. The trend of ketone was the same as that of estriol, and the increase of 5-month-old was the highest at 6 months. The 7-month-old decreased rapidly, and the age of 8 months increased to the maximum value, and then decreased gradually. Testosterone 3 - 6 months of age rose slowly, 6 - 9 months of age decreased slowly, 9 - month - old content reached the minimum, after the rapid rise in 11 months to reach the maximum; Whitmania pigra progesterone in addition to 1,4,9,11 Four months are outside the larger, 10 months of age for the maximum, 11 months of rapid decline to a minimum. Conclusion: 6 months of Whitmania pigra lethal estrogen content reached its maximum, 10 months of Whitmania pigra progesterone content reached its maximum, 10-11 months of Whitmania pigra male hormone rapid rise to the maximum, Whitmania pigra steroid hormone synchronization in the gonadal differentiation.
Key words：Whitmania pigra; steroid hormones, reproductive cycle changes, Gonadal differentiation
目录
摘要 1
关键词： 1
Abstract 1
Key words 1
引言 1
1 材料与方法 2
1.1实验材料和试剂 2
1.2 仪器 2
1.3 取样方法 2
1.4 激素测定方法 2
1.4.1 实验提取步骤 3
1.4.2 液相条件 3
1.4.3 质谱条件 3
1.5数据处理方法 4
2 结果与分析 4
2.1生殖周期雌激素的变化 4
2.2生殖周期雄激素的变化 4
2.3生殖周期孕激素的变化 5
2.4生殖周期四种激素的变化 5
3 讨论 5
3.1性类固醇激素与性腺发育 5
3.2雌雄同体与性腺发育 6
3.3小结 6
致谢 6
参考文献： 7
蚂蟥生殖周期性类固醇激素变化的初步研究
引言
蚂蟥为环节动物门蚂蟥科动物蚂蟥（Whitmania pigra Whitman）的干燥全体[1]，又称宽体金钱蛭[2]随着市场的发展，中成药及保健产品中以蚂蟥为原料的药品和保健品日渐增多,蚂蟥的野生资源日益枯竭,导致蚂蟥的价格不断飙升，因此蚂蟥的人工养殖工作已受到业界高度重视。