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摘要：本试验旨在进一步探索长期灌胃添加低剂量纳米ZnO对小鼠发育及免疫功能的作用。试验分为三组：1）对照组，灌胃生理盐水；2）灌胃每千克体重25mg纳米ZnO（以锌计）；3）灌胃每千克体重100mg纳米ZnO（以锌计），每组三个重复，每个重复8只21天断奶仔鼠。13周结束，分别从生长、免疫等方面系统评价长期添加低剂量纳米ZnO的安全性。结果表明：长期灌胃没千克体重25mg及100mg纳米ZnO对小鼠的生长无显著影响 (P＞0.05)；可显著增加十二指肠绒毛的高度及隐窝深度(P<0.05)以优化肠形态。在元素沉积方面，灌胃添加每千克体重25mg及100mg 纳米ZnO均可显著增加肝脏中Zn的含量及肾脏中Fe的含量(P <0.05)，对肌肉中元素沉积均无显著影响。此外，灌胃100mg每千克体重纳米ZnO显著增加肾脏中Fe的含量并且使血液内淋巴细胞数显著增多(P <0.05)。本研究结果提示，长期灌胃添加每千克体重25mg和100 mg 纳米ZnO对小鼠不仅无明显毒性作用，而且在肠形态及免疫方面存在可能的促进作用。29798
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毕业论文关键词：纳米ZnO；小鼠；毒性；生长；免疫
Effects of Nano Zinc Oxide on Growth Performance and Immunity in Mice via Long-Term Intragastric Administration
Abstract：This study aimed at further investigtating effects of low dose nano Zinc Oxide (nano-ZnO) on the growth and immune function of mice via long-term intragastric administration for 13 weeks. The experiment was pided into three groups randomly, which were 1)the control group, treated with physiological saline; 2)25mg/kg nano-ZnO group: intragastric administration with 25 mg zinc as the form of nano zinc oxide /kg body weight; 3) 100mg/kg nano-ZnO group: intragastric administration with 100mg zinc as the form of nano-ZnO /kg body weight. Each group contained 3 repeats, eight mice for each repeat. All mice in the experiment weaned at 21 days. After 13 weeks of experiment, the safety of long-term low dose nano-ZnO was evaluated from the aspects of growth, immunity and so on. The results showed that the gastric 25 and100 mg/kg body weight of nano-ZnO had no significant effect on the growth of mice (P＞0.05); optimized intestinal morphology by enhancing the height of duodenal villus and the crypt depth significantly (P <0.05).In terms of element deposition the gastric 25 and 100mg/kg body weight of nano-ZnO significantly increased the content of Zn in liver and the content of Fe in kidney and showed no effect on element deposition in muscle. In addition, the gastric 100mg/kg body weight of nano-ZnO significantly increased the content of Fe in kidney and the number of lymphocytes in the blood (P < 0.05).Our results demonstrated that the long-term gastric 25 and 100mg/kg body weight of nano-ZnO has no obvious toxic effect, but it can probably promote intestinal morphology and immunity.
Key words: zinc oxide nanoparticle; mice; toxicity; growth; immunity
目 录
摘要3
关键词3
Abstract3
Key words3
1.引言3
2.文献综述4
2.1纳米材料 4
2.1.1纳米材料的特性4
2.1.2 国内外研究进展4
2.2纳米氧化锌及其安全性研究4
2.2.1纳米氧化锌的特性及应用4
2.2.2纳米氧化锌的安全性评价4
2.3亟待解决的问题4
3.材料与方法5
3.1试验材料5 3.1.1主要试剂5
3.1.2主要仪器5
3.1.3试剂配制5
3.2试验设计5
3.3试验对象5
3.4饲养管理5
3.5样品采集和指标测定5
3.5.1生长性能（体重）评估5
3.5.2血清生化指标测量6
3.5.3十二指肠制片、观察6
3.6数据处理分析6
4.结果与分析6
4.1长期灌胃添加低剂量纳米ZnO对对小鼠生长性能（体重）的作用6
4.2长期灌胃添加低剂量纳米ZnO对对小鼠血清生化的作用6
4.3长期灌胃添加低剂量纳米ZnO对小鼠十二指肠结构的作用7