摘要：目的:研究甘草酸(Glycyrrhizic acid)对急性四氯化碳(CCl4)肝损伤小鼠的保护作用及其机制。方法:采用CCl4诱导的小鼠急性肝损伤模型,分别设空白组,实验组。测血清中甘草酸的含量,观察肝损伤后小鼠对甘草酸的代谢的变化。方法：先建立肝损伤模型，取6只小鼠，雌雄各半，分2组，每组3只，0.3%四氯化碳腹腔注射.再继续三天灌喂甘草酸，最后一次灌胃后每半小时活体眼球取血，取小鼠血液，立即4000转/分，离心10min，保留上清血清。利用高效液相分析不同时间其在体内的含量。结果：通过比较正常小鼠和肝损伤小鼠对甘草酸的代谢得到肝损伤的小鼠比正常小鼠代谢变慢。甘草酸对于在体内的代谢雌雄差异不大。69057

毕业论文关键词： 甘草酸 四氯化碳 急性肝损伤 药物代谢动力学

Study on the effect of the drug on the pharmacokinetics of the rat liver after liver injury

Abstract:Objective: To study the protective effect and mechanism of GA on acute carbon tetrachloride (CCl4) - induced liver injury in mice.method:Acute liver injury model induced by CCl_4 in mice,Set up the blank group and the experimental group respectively.To observe the content of serum in the serum, and observe the changes of the metabolism of the mice after liver injury.Methods: liver injury model was established,From 6 mice, male and female, were pided into 2 groups, 3 rats in each group, 0.3% by intraperitoneal injection of carbon tetrachloride. Continue for three days fed with glycyrrhizic acid,Last time after intragastric administration of every half hour in vivo eyeball blood from mice blood, immediately 4000 rpm, 10min, maintain supernatant serum. Using high performance liquid phase analysis of different time its content in vivo.Results: by comparing the normal mice and liver injury mice, the metabolism of anthocyanins in mice with liver damage was slower than that of normal mice.The difference of the metabolism of the male and female in the body is small.

[Key words]：GA；CCl4；mouse;liver injury；Pharmacokinetics

目录

引言 1

1 材料与方法 1

1.1 材料 1

1.1.1 药品 2

1.1.2试剂 2

1.1.3仪器与设备 2

1.1.4实验动物 2

1.2方法 2

1.2.1 配制试剂： 2

1.2.2诱导的小鼠急性肝损伤模型建立 3

1.2.3甘草酸药物处理 3

1.2.4 活体眼球取血 3

1.2.5 分离血清 4

1.2.6 HPLC检测指标及方法 4

2 实验结果与分析 5

2.1实验过程记录 5

2.1.1 待肝损伤小鼠体重 5

2.1.2 肝损伤建模时所需药量（0.1ml/10g） 5

2.1.3 肝损伤小鼠每次甘草酸灌胃所需药量 （0.2ml/10g） 5

2.1.4 每次眼球取血点 6

2.2一般情况观察 6

2.3甘草酸标准曲线的绘制 6

2.5灌胃后血中甘草酸的动力学变化 8

2.6不同时间血中甘草酸含量的性别差异