群体感应自杀种群在富营养培养基中的生长模型
关键词：群体感应；细菌自杀；诱导剂 ；富营养培养基
The growth model of suicide population with quorum sensing in rich nutrient medium
Abstract: Suicide behavior of bacteria is a valuable natural phenomenon. To generate and maintain the appearance of the phenomenon and its effects on bacteria group evolution of the research is of great significance both in theory and in practice. This topic is based on the original research foundation, to top10F 'e. coli strains, in LB liquid medium cultivation, using IPTG as inducers, quorum sensing suicide phenomenon in the bacteria, comparing bacterial growth curve under different growth conditions. IPTG as external inducers of suicide phenomenon induced by bacteria, under the condition of temperature and PH value of the same, IPTG concentration is higher, the suicidal behavior, the more obvious; AHL is one of the important factors of bacterial suicidal behavior, at higher temperature, PH value under the condition of high AHL easy to decompose, reduced stability. So in same IPTG concentration, PH value at the same time, the training of the lower temperature, suicidal behavior, the more obvious; And IPTG concentration, same incubation temperature and same culture medium, the lower the PH, suicidal behavior, the more obvious. By comparing the experiment found that in the PH = 5.7 LB liquid medium add IPTG concentration of 1 mm, under 30 ℃ cultivate bacteria to suicide phenomenon is most obvious.
Keywords: Quorum Sensing; Bacteria suicide; inducer; Rich nutrient medium
目录
1 绪论 1
1.1 细菌的自杀行为 1
1.2 细菌的群体感应现象 1
1.3 自杀基因回路的构建及其意义 2
1.4 国内外研究现状与发展趋势 4
1.5 本课题研究的内容和意义 4
2 实验方法 5
2.1 拟使用的材料试剂、仪器设备 5
2.2 拟采取的方法 5
2.3 拟定的实验方案 5
3 实验结果与讨论 7
3.1 比较无IPTG与有IPTG时，不同温度下细菌的生长状况 7
3.1.1 比较无IPTG时，不同温度下细菌的生长状况 7
3.1.2 比较有IPTG时，不同温度下细菌的生长状况 9
3.2 比较30℃时，不同pH值与IPTG浓度对细菌自杀情况的影响 10
3.2.1 比较pH值相同时，IPTG浓度对细菌自杀情况的影响 10
3.2.2 比较IPTG浓度相同时，不同pH值对细菌自杀情况的影响 12
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