水中亚硝酸盐的微生物处理方法研究
毕业论文关键词： 乳酸菌 ；亚硝酸钠 ；固定化 ；包埋 ；海藻酸钠
Study on the treatment of nitrite in water by microbial method
Abstract:With the rapid development of industry and agriculture, groundwater in many countries have been contaminated nitrite, and more serious, and the water will co nitrite animals produce many hazards. And this paper is how to fix the immobilized cell technology efficiently thereby degrading microbial flora of sodium nitrite. Research the effects of immobilized cells under different conditions on the embedding of nitrite degradation. Many experiments show that: the Lactobacillus bacteria as sodium nitrite degradation, then the optimal conditions alginate immobilized cells nitrite degradation embedding method is: 2.5% sodium alginate, calcium chloride 1.5% of the 3rd type needle selection (particle size), and at this time the gel beads having a good flexibility and proliferative conditions, immobilized cells can be reused, the experiment was repeated four times.
Keywords:lactic acid bacteria; sodium nitrite; immobilization; embedding; sodium alginate
目录
1前言 1
1.1 亚硝酸盐概述 1
1.2 亚硝酸盐国内外研究现状 1
1.3 乳酸菌的固定化技术概述 2
1.4 乳酸菌的包埋技术 2
1.5 本课题的研究目标和意义 3
1.6 本课题研究的主要内容 3
1.6.1 掌握菌种的生长情况并显微镜观察其形态 3
1.6.2亚硝酸盐降解菌的发酵条件实验 3
1.6.3 确定细胞最佳包埋条件 3
1.6.4 固定化细胞处理亚硝酸盐实验 3
1.6.5 活菌测定 3
1.6.6 固定细胞的收集 3
2 实验材料与方法 4
2.1 实验原材料 4
2.2 主要化学试剂 4
2.3 主要仪器和设备 5
2.4 实验方法 5
2.4.1 培养基的配置 5
2.4.2菌体生长曲线的绘制 5
2.4.3革兰氏染色观察 6
2.4.4 亚硝酸盐降解菌的发酵条件实验 6
2.4.5 细胞的固定化技术 6
2.4.6 细胞最优包埋条件的确定 6
2.4.7 发酵液亚硝酸钠含量测定 7
2.4.8活菌测定 8
3.结果与讨论 9
3.1菌体的生长情况 9
3.2乳酸菌的活菌计数与显微镜观察 9
3.3发酵液中亚硝酸钠的含量测定 11
3.4细胞固定化技术的方法优化 11
3.4.1 海藻酸钠浓度对固定化细胞降处理亚硝酸钠的影响 11
3.4.2 氯化钙溶液浓度对固定化细胞处理亚硝酸钠的影响 12
3.4.3 粒径大小对固定化细胞处理亚硝酸钠的影响 13
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