摘    要  本实验以草莓（Fragaria ananassa Duch）匍匐茎顶芽茎尖为外植体进行草莓茎尖脱毒培养研究，包括不同BA浓度对草莓外植体芽增殖的影响以及IBA对草莓外植体诱导生根的影响。对外植体生长发育情况定期进行观察记录，比较其生长状况。实验结果表明，当BA浓度分别为0。25 mg/L，0。5 mg/L，1。0 mg/L时的MS培养基，对外植体平均株高没有多大影响；当BA浓度为0。25 mg/L时，对于外植体芽数没有优势但芽势十分健壮；而BA浓度为1。0 mg/L时，对株高没有优势但芽诱导频率增加。综合来看，当BA的浓度为0。5 mg/L时，对草莓外植体芽增殖更有优势;在研究IBA对外植体诱导生根影响时，加IBA比不加者对外植体生根更为有利，更易成活。85380

This experiment was to use the Fragaria ananassa Duch stolons crown to reseach the virus-free culture of Fragaria ananassa Duch’s stem tip, including the influence of different concentration of BA Fragaria ananassa Duch explant bud proliferation, as well as the effects of different IBA concentration on Rooting of Fragaria ananassa Duch。  The growth status was compared by recording the  growth and development situation of explant。 Experimental results show that when the concentration of BA was 0。25 mg/L, 0。5 mg/L, 1。0 mg/L of culture medium respectively, on the bud proliferation, there was no effect on seedling high; but the concentration of BA was 0。25mg/L, it isn’t suitable for the proliferation of the average number of bud but the bud grows very strong and healthy; when the concentration of BA was 1。0 mg/L, it hadn’t the seedling height advantage but it is suitable for the proliferation of the average number of bud; Taken together, when the concentration of BA was 0。5mg/l, it was appropriate for the average number of bud; When Studying on the effects of different IBA concentration on Rooting of Fragaria ananassa Duch, we found add IBA to Fragaria ananassa Duch was most favorable for rooting 。 

毕业论文关键词：草莓；茎尖；脱毒苗；芽增殖；生根源Q于W优H尔J论K文M网WwW.youeRw.com 原文+QQ75201.,8766

Keyword: Fragaria ananassa Duch；Stem tip；virus-free seedling；bud proliferation；Rooting

目    录

引言 4

1材料和方法 5

1。1 实验材料 5

1。2 方法 5

1。2。1 脱毒茎尖的诱导培养 5

1。2。2 继代培养及不同浓度BA对草莓外植体芽增殖的影响 6

1。2。3 未加IBA与加IBA对草莓外植体诱导生根的影响结果比较 6

2结果与分析 7

2。1 BA对草莓外植体芽增殖的影响 7

2。2 IBA对草莓外植体诱导生根的影响 8

3讨论 9

3。1 研究意义 9

3。2 不同BA浓度对草莓外植体芽增殖的影响 9

3。3 IBA对草莓外植体诱导生根的影响 9

4 展望 10

参考文献 10

致谢 10

引言来自优W尔Y论W文C网WWw.YoueRw.com 加QQ7520,18766

草莓（Fragaria ananassa Duch）又称地桃、土多啤梨等，是蔷薇科（Rosaceae）草莓属（Fragaria）植物，高约30cm，有匍匐枝，枝条细长。茎密被开，展黄棕色柔毛，几乎与叶等长。草莓营养价值高，含多种营养物质且具有保健功效，以及其较高的经济价值现已被多个国家和地区引种。我国种植草莓的时间并不长，目前产量较多的有沈阳、杭州等市。近年来，随着经济水平的不断提高，人们对于自身健康愈发重视，草莓因其独特的口感与功效以及逐渐多元化的种植模式，获得了广大消费者的喜爱，在国内的需求愈加旺盛。