摘  要:采用中心复合试验设计(CCD)和响应曲面的方法，研究了密度（0。1g/L、1。05g/L、2。0g/L）、光照强度（1000lux、2000lux、3000lux）以及光照周期（06L:18D、15L:09D、24L：00D）对卤虫休眠卵孵化率的联合影响。结果表明：密度与光照周期的一次效应和二次效应对孵化率均有显著影响（p<0。05），随着密度的增大，休眠卵的孵化率呈先上升后下降的趋势；在试验光照周期范围内，曝光时间越长，孵化率越高；密度与光照周期之间存在着极显著的交互作用（p<0。01）,1。05g/L和24L:00D的密度和光照周期在孵化百分比方面具有良好的孵化效果。光照强度一次效应和二次效应对卤虫孵化率的影响均不显著。响应面法对试验因子和孵化率的二次多元回归方程进行拟合，方程决定系数为96。21，表明方程拟合度好可用来预测，推荐最佳优化条件组合为密度1。12g。L-1,光照强度为1598lux、光照周期为23L:01D，孵化率可达96。56%，可靠性达91。35%。94763

毕业论文关键词：卤虫休眠卵、孵化率、密度、光照强度、光周期、响应面分析

Abstract：By adopting central composite design(CCD) and response surface methodology(RSM) this experiment studied the combined effects of density (0。1g/L、1。05g/L、2。0g/L), light intensity (1000lux、2000lux、3000lux) and photoperiod(06L:18D、15L:09D、24L：00D) on the hatching rate of Artemia parthenogentica cysts。 The results show that the effects of primary and secondary effects of density and photoperiod on the hatching rate were significant。 With increasing density the hatchability decreased after。 In the experiment photoperiod range, the longer the exposure time, the higher of hatching rate。 There is a significant interaction between density and photoperiod for hatching rate of Artemia parthenogentica cysts。 Density and photoperiod have significant effects on hatching of cysts。 The density of 1。05g/L and photoperiod of 24L:00D have good results in hatching percentage and efficiency of cysts。 The linear and quadratic effects of light intensity were not significant。 The response coefficient is 96。21, which indicates that the fitting degree of the equation can be used to predict the optimal optimization conditions。 The combination of the optimum conditions for germination were density of 1。12g。L-1light intensity of 1598lux and photoperiod of 23L:01D, under which the hatchability reached 96。56% and the reliability peaked to 91。35%。

Keywords：Artemia parthenogentica cysts, hatchability, density, light intensity, photoperiod, response surface methodology

目   录

1。前言 5

2 材料与方法 5

2。1 材料 5

2。2 试验方法 5

2。3 孵化 6

2。4 试验设计 6

2。5 孵化率测定方法与孵化率计算 7

2。6 数据结果处理 7

3 结果与分析 7

3。1 密度与光照强度对卤虫休眠卵孵化率的影响 8

3。 2 密度与光照周期对卤虫休眠卵孵化率的影响 8

3。3 光照强度与光照周期对卤虫休眠卵孵化率的影响 8

3。4 孵化条件拟合优化 8

3。5 预测结果验证 9

4 讨论 12

4。1 密度、光照强度与光照周期的联合影响