球囊霉素对土壤中多环芳烃吸附的影响
毕业论文关键词：球囊霉素；多环芳烃；土壤；吸附
Effects of Glomalin on the Adsorption of Polycyclic Aromatic Hydrocarbons in Soils
Abstract：In order to explore the mechanism of action of glomalin-related soil protein (GRSP) and polycyclic aromatic hydrocarbons (PAHs). In this paper, the effect of glomalin on the adsorption of PAHs in soil was studied by using phenanthrene as representative of PAHs, in addition,Easily Extractable Glomalin (EEG) and Total Glomalin (TG)was compared . The soil samples were collected from mollisols,paleudult and typic paleudalf. The results showed that glomalin-related soil protein (GRSP) was extracted from the Zijin Mountain bush soil in Nanjing.Then,using pH 7.0, 20mmol•L-1 and pH 8.0, 50mmol•L-1respectively,sodium citrate solution to extract GRSP, with the extracted glomalin to do the adsorption test. The results showed that the addition of Easily Extractable Glomalin could inhibit the adsorption of phenanthrene effectively in the experimental condition, and the inhibitory effect was enhanced with the increase of the concentration of Easily Extractable Glomalin, also, the effect of total gliomalin (TG) on the adsorption of PAHs in soil was the same .The adsorption of phenanthrene in the soil was closely related to the content of organic matter in the soil. The content of DOM in the solution was significantly inhibited by the soil, and the inhibitory effect was more obvious with the increase of DOM concentration.
Key words: glomalin；polycyclic aromatic hydrocarbons；soil；adsorption
目 录
摘要 1
关键词 1
Abstract 1
Key words: 1
引言 2
1材料与方法 2
1.1 供试材料 2
1.1.1供试土样 3
1.1.2球囊霉素 3
1.2试验方法 4
1.2.1吸附实验 4
2结果与讨论 5
2.1土壤对菲的等温吸附曲线 5
2.2球囊霉素（GRSP）对于土壤中菲吸附的影响 6
2.3不同浓度球囊霉素对DOM的影响 8
3讨论 10
3.1土壤吸附菲的主导机制 10
3.2球囊霉素（GRSP）有效抑制土壤对菲的吸附 10
3.3 DOM对土壤吸附菲的影响 10
致谢 11
参考文献 11
球囊霉素对土壤中多环芳烃吸附的影响
引言
1996年美国马里兰大学的Wright等[1,2]在丛枝菌根真菌（AMF）菌丝表面找到了一种不明蛋白，它可以与单克隆抗体（MAb32B11）发生免疫性特异反应，并且这种蛋白性质十分稳定，不溶于水，只能通过使用柠檬酸钠溶液（中性至碱性）并置于121℃高温条件下才可以将其提取出来。Wright[2]等人做了进一步的实验，他们从美国的亚特兰大州未经耕作的酸性土壤中，于121℃高温条件下用20mmol•L-1的柠檬酸钠 溶液 提 取出了一种蛋白质，他们把这类含量丰富特殊的蛋白质与另一种从丛枝菌根真菌中提取得到的蛋白质进行了聚 丙烯 酰 胺凝 胶电 泳（SDS-PAGE）分析比较，结果表明两者的条带基本完全一致。研究继续深入，他们发现一开始把这类从土壤里提取出的不明蛋白认作丛枝菌根真菌分泌的基因蛋白，但却忽视了此不明蛋白中是不是还有其他成分，因此用球囊霉素命名好像有点片面，之后，Rillig[3]与Lovelock等[4]把它命名为球囊霉素相关土壤蛋白（glomalin related soil protein，GRSP），将原先命名（球囊霉素）取而代之。Driver等[5]发现，球囊霉素并不是通过直接释放的方式进入土壤里，而是紧密结合于菌丝的细胞壁与真菌孢子上，并且伴随两者的衰亡与降解过程而进入到土壤里。自然状态下的球囊霉素性质十分稳定，而且土壤中含有丰富的球囊霉素。有研究表明，球囊霉素是土壤最为重要的碳库之一，是促进土壤团聚体形成的重要粘合剂，此外，土壤中大量存在的球囊霉素拥有能够密封AMF菌丝、阻止菌丝体内的营养物质流失的功效，对于促进土壤团聚体(soil aggregate)的形成、文持土壤稳定性以及改善土壤性能等发挥着及其重要的作用。近年来，球囊霉素在土壤中的作用已经成为土壤领域研究的热点。