北京鸭盲肠微生物区系分析
毕业论文关键词:北京鸭；肠道微生物群；盲肠。
The analysis of Peking duck cecum gut microflora
Abstract: Duck gut microbiota has important roles in host performance, health and immunity. Understanding the topological difference in gut microbial community composition is crucial to provide knowledge on the functions of each members of microbiota to the physiological maintenance of the host. This study used High-throughput sequencing technologies to analyze the microbiota fauna of the duck cecum. The cecum used is from five three weeks Peking ducks which is fed normally. After the extraction of total DNA samples, we synthetized primers according to the V3 + V4 hyper-variable region of 16S rRNA .Then we conducted PCR amplification and the purification of the PCR product. Next, we formed the sequencing library. After sequencing, sequence optimization, OUT clustering, analysis of classification and abundance of species persity, single sample analysis, analysis of the persity of many samples, we can get 1447 OUT totally, and find that the species composition of samples and proportion in all samples is different: From the classification level in Phylum, we can find that bacteroidetes is one of the most abundant species (55% -74%); From the classification level in Genus, we can find that bacteroides is one of the most abundant species (43% -61%). And we learn that different duck gut microbes have obvious difference between function and role. Finally, we can conclude that from the phylum to genus, as the more precise classification standard, we can detected the more microbial flora of the category. The ratio of bacteroides still represents a significant proportion. Based on the composition of the microbial community, future gut modulation with beneficial bacteria such as probiotics may benefit the host.
Key words: Peking duck; Gut microbiota; Caecum.
目录
引言 1
1 材料与方法 1
1.1实验流程图 1
1.2信息分析流程 2
1.3鸭和采样 3
1.4 DNA的提取 4
1.5 16S rRNA 高变区的 V3+V4 区域基因扩增和Illumina测序 4
1.6 生物信息学分析 4
2 结果 5
2.1 测序数据质控 5
2.2 序列优化 5
2.2.1 Reads 拼接成 Tags 5
2.2.2 Tags 优化及统计 6
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