苟池盐湖嗜盐细菌多样性研究
毕业论文关键词：盐湖；嗜盐细菌；16S rRNA基因；454高通量测序；多样性
Bacteria Diversity Research of GouChi Salt Lake
Abstract: The study is based on 16S rRNA gene analysis and PCR in halobacteria of Gouchi salt Lake, using 454 high throughput sequencing methods to detect the gene sequencing and optimize the valid reads obtained. After searching in silva database, using taxonomic methods to analy bacterial species composition and halobacteria persity of Gouchi salt lake. It turns out that 33840 valid cloning reads have been obtained, and there have been 31329 trimmed reads after optimizing. 27.08%（8483）reads cannot be classified to any phylum-level taxon and 183 OTUs were generated by sequence alignment. The bacterial community in the sample was dominated by Proteobacteria, Bacteroidetes and Firmicutes phylum, Cytophagia, Sphingobacteriia and Gammaproteobacteria class, Rhodothermaceae, Chitinophagaceae and Ectothiorhodospiraceae family, Salinibacter, Chitinophagaceae_uncultured, Spiribacter, Halomonas and Lactococcus genus. The study of the bacterial structure and species persity of the salt lake can help us to know the basic characteristics of brine in local environment, and provide data support for species exploitation in the future.
Key words: Salt lake; halobacteria; 16S rRNA gene; Biopersity; 454 pyrosequencing
目录
引言 1
1 材料与方法 2
1.1材料 2
1.1.1样点描述 2
1.1.2水样采集 2
1.1.3主要仪器与试剂 2
1.2实验流程与方法 3
1.2.1宏基因组DNA提取 3
1.2.2宏基因组电泳分析 3
1.2.3 16S rRNA的PCR扩增和胶回收 4
1.2.4荧光定量 4
1.2.5 454高通量测序及相关数据处理 4
1.2.6 OTU聚类分析 5
1.3相关指数与曲线说明 5
1.3.1 稀释性曲线 5
1.3.2 多样性指数 5
2 结果与分析 5
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