独角金内酯(SL)对菊花蚜虫取食行为的影响
毕业论文关键词：菊花；独角金内酯；抗蚜性
The effects of strigolactone on feeding behavior of aphids on chrysanthemum
Abstract：Chrysanthemum (Chrysanthemum morifolium), one of ten traditional Chinese famous flowers and one of four cut flowers over the world, of large market share and high ornamental and economic values. During the whole cultivated progress, aphids destroy chrysanthemum easily and universally, causes serious economic loss. The roles of strigolactone (SL) in stress tolerance in reported recently. Research of SL in resistance of chrysanthemum to aphid is essential to control aphids and non-chemical cultivation. We set up to make ‘shenma’, an aphid-sensitive variety as our research object. We used CmCCD8 over-expression ‘shenma’ lines and GR24 treated ‘shenma’ plants as material, performed aphid inoculation assay, olfactometer bioassay and electrical penetration graph(EPG) to illustrate the effects of SL on feeding behavior of chrysanthemum aphids. The results we found are as follows: GR24 treatment enhanced the aphid density compared with control plants and CmCCD8 over-expression lines are significant high. Electrical penetration graph (EPG) shows that the feeding activities of the tested aphids on the CmCCD8-OX plants differed from those on the WT plants. The time to the first cell puncturing (pd), the total duration of the non-puncturing phase (np) and the time to the first pathway phase (c) in both CmCCD8-OX plants were shorter than those in the WT plants, and the total duration of the pathway phase in CmCCD8-OX plants was longer than in WT plants significantly. GR24 treated plants and transgenic lines play negative roles in the response of chrysanthemum to aphids, which indicates SL has the function on aphids’ sensitivity.
Key words: Chrysanthemum; strigolatone; aphid
目 录
摘要1
关键词1
Abstract1
Key words1
引言2
1 材料与方法 3
1.1 材料 3
1.1.1 植物材料3
1.1.2 化学试剂3
1.2 方法 3
1.2.1 外源GR24处理3
1.2.2 蚜虫接种试验3
1.2.3 蚜口数目统计3
1.2.4 蚜虫取食刺吸电位(EPG)检测3
1.2.5 “Y”型嗅觉仪——蚜虫趋避性试验3
2 结果与分析3
2.1 不同浓度GR24处理对菊花蚜虫增殖的影响3
2.2 CmCCD8转基因植株的抗蚜性鉴定4
2.3 CmCCD8转基因植株的蚜虫取食刺吸电位（EPG）图谱5
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