环境水平下四环素对蓝藻生长及藻毒素释放的影响研究
毕业论文关键词： 四环素；蓝藻；毒性；藻毒素；生长抑制
The effects of tetracycline at environmental level on growth and microcystins release in cyanobacterium Microcystis aeruginosa
Abstract： The problem of environmental pollution caused by the use of antibiotic substancehas attracted global attention.China is a big country of consumption of antibiotics, The problems of antibiotic pollution needs to be solved.The study of the influence of tetracyclineon the growthofcyanobacteria andmicrocystin release is very lack. Therefore, the study on the toxicity of tetracycline on cyanobacterial growth andthe release of algal toxins can not only help us to further understand the chemical properties of tetracycline and biological effectiveness, but also can further understand the characteristics of cyanobacteria.This paper introduced the ecological toxicity of tetracycline antibiotics,using Microcystis aeruginosa as the research object. Effects of tetrecycline on the growth of Microcystis aeruginosa, and the protein content, chlorophyll content analysis, oxidative stress and effects of microcystinsrelease, and aquatic ecological environment safety evaluation of tetracycline were studied.
KeyWords：Tetracycline;Blue-green algae; Toxicity; Algae toxin; Growth inhibition
目录
1. 引言（绪论） 3
1.1蓝藻和藻毒素 3
1.2 抗生素的污染现状 5
1.3 四环素类抗生素的污染现状 5
1.4 四环素类抗生素的生态毒性 6
1.4.1 四环素类抗生素对土壤生物的生态毒性 6
1.4.2 四环素类抗生素对植物的生态毒性 6
1.4.3 四环素类抗生素对动物的生态毒性 7
1.4.4四环素类抗生素对水体的生态毒性 7
1.5四环素的理化性质 8
1.6 课题研究的意义和目的 9
1.7 课题研究的内容 9
2. 实验条件及预实验 10
2.1 实验条件 10
2.1.1 实验仪器 10
2.1.2 实验材料 10
2.1.3 实验药品 12
2.2 预实验 12
2.2.1 BG11培养液的配置 12
2.2.2 高压蒸气灭菌 12
2.2.3 蓝藻的接种 13
2.3 实验方案论证 13
3. 实验方案与方法 14
3.1 四环素储备液的配置 14
3.2 蓝藻细胞数与吸光度的关系 14
3.2.1 实验原理 14
3.2.2 实验方法 15
3.3 蓝藻生长曲线及抑制率 15
3.4 考马斯亮蓝染色法测定蛋白质含量 15
3.4.1 实验原理 15
3.4.2 试剂配制 16
3.4.3 实验方法 16
3.5叶绿素含量的测定 16
3.6抗氧化损伤的测定 17
3.7微囊藻藻毒素的测定方法 17
下一篇：MS月季‘真宙’的组织培养研究