蒿属植物及其近缘种挥发性成分分析
毕业论文关键词：菊科植物;叶片; GC-MS;萜类化合物;化学分类
GC-MS analysis of the volatile constituents of Artemisia and its related species
Abstract: The green organs, especially the leaves, of manyCompositae plants possess characteristicaromas. The constituents, mainly volatile compounds, in the leaves of 14 scented plant materials were qualitatively and quantitatively compared via gaschromatography-mass spectrometry (GC-MS). A total of 213 constituents were detected and tentatively identified in the leaf extracts, and terpenoids (especially monoterpene and sesquiterpenederivatives), accounting for 40.45%–90.38% of the total compounds, were the main components in most of extracts. The quantitative resultsrevealed perse concentrations and compositions of the chemical constituents between species. Principal component analysis (PCA) showed that different groups of these Compositae plants were characterized by main components of α-thujone, germacrene D, eucalyptol, β-caryophyllene and camphor, for example. Hierarchicalcluster analysis (HCA) based on the terpenoid composition was also used to classified the tested species. The cluster memberships corresponding to molecular phylogenetic framework, as well as the linkages of Chrysanthemumindicumvar. aromaticumvs. C. indicum,and Opisthopappus longilobusvs. O. taihangensis, suggested that terpenoid compounds have a potential to be used for thechemotaxonomy of these plants with a genetic relationship. Ultimately, these results provide a phytochemical foundation for the usage of these scented Compositae plants.
Key words: Compositae plants; leaves; GC-MS; terpenoids; chemotaxonomy
目录
摘要 3
关键词 3
Abstract 3
Key words 3
引言 3
1材料与方法 5
1.1植物材料 5
1.2 样品制备 5
1.3 GC-MS条件 5
1.4峰值识别 5
1.5统计分析 6
2结果与分析6 6
2.1 挥发性成分鉴定和定量分析 6
2.2 化合物类型的比例 7
2.3主成分分析和聚类分析 8
3讨论 10
致谢 12
参考文献 13
表1 试验所用14种菊科植物 5
表2 14种叶片样品挥发性物质类别数量 6
图1 14个样品挥发性成分含量图 7
图2 14个样品叶片挥发性组成的相对含量 8
图3 14个样本的主成分分析 9
图4 萜类化合物相对含量的14个样品的层次聚类分析的树状图 10