脱落酸对葡萄果实着色代谢的影响机理
毕业论文关键词：葡萄果实；ABA；Abz；NDGA
The effects of ABA on fruit coloring mechanism in grape berries
Abstract: The effects of ABA, Abz (Abscinazole, an inhibitor of CYP707A), and NDGA (Nordihydroguaiaretic acid, an inhibitor of NCED) applications on fruit firmness, anthocyanin biosynthesis, aroma volatiles, and their related genes were investigated in grape berries (Vitis vinifera L. × Vitis labrusca L. cv. Kyoho). ABA or Abz application promoted anthocyanin, aroma volatile productions, but reduced fruit firmness and PG, PME and cellulase activities. Whereas NDGA showed an opposite effect. The expressions of VvMYBA2, VvUFGT or VvEGS1 coincided with anthocyanin concentration or aroma volatiles production, except that for the VvLAR1. Endogenous ABA concentrations and the expressions of VvOPR3, VvACO1, and VvSUT11 were increased by ABA and Abz applications, but decreased by NDGA application. The expressions of VvNCED1, VvCYP707A1, VvPYL8 coincided with endogenous ABA concentrations. In contrast, VvCYP707A1 expression was decreased by Abz application. The expressions of VvPP2C49 showed opposite changes to ABA concentrations. These results suggest that endogenous ABA concentrations influence the anthocyanin formation, aroma volatile production, fruit softening, and sugar metabolism through the expressions of VvMYBA2, VvUFGT, VvEGS1 and VvSUT11.
Key words: Grape fruit ；ABA ； Abz ；NDGA
目 录
摘要 3
关键词 3
Abstract 3
Key words 3
引言 3
1 材料与方法 5
1．1 植物材料 5
1．2 方法 5
1．2．1 RNA的提取与定时荧光定量PCR分析 5
1．2．2 花青苷、ABA、挥发性物质浓度、可滴定酸、可溶性固形物以及硬度的测定 6
1．2．3 细胞壁代谢酶活性 6
2 结果与分析 6
2．1 不同处理（ABA、Abz、NDGA）对ABA合成代谢基因表达水平的影响 6
2．2 不同处理（ABA、Abz、NDGA）对花青苷合成的影响 7
2．3 不同处理（ABA、Abz、NDGA）对成熟相关基因表达水平的影响 8
2．4 不同处理（ABA、Abz、NDGA）对葡萄果实生理变化的影响 9
3 讨论 10
致谢 12
参考文献 13
脱落酸对葡萄果实着色代谢的影响机理
引言
葡萄(Vitis vinifera L.)是一种多年生落叶木质藤本植物，是世界上栽培历史最长、产量最大的果树之一[1]，也是我国的主栽果树之一。葡萄为世界四大水果之一，如何提高其产量和品质一直是葡萄生产和科学研究的重点[2]。色泽是果实重要的品质特征，果实色泽和果实品质是决定鲜食葡萄商品价值和市场竞争力的重要指标。随着生活水平的日益提高，人们对水果的要求不仅仅是营养丰富了，而且对其外观也提出了更高的要求。果皮的着色程度也会直接影响到葡萄的营养价值和加工特性。如何使葡萄提前成熟着色并提高其品质一直是科学研究的重点。
上一篇：不同水稻品种氮素利用效率的评估
下一篇：钾与梨树营养和果实品质的关系研究