一种水稻F-BOX蛋白的生物信息学分析
毕业论文关键词： 水稻；F-BOX 蛋白；泛素蛋白途径；生物信息学分析
The Bioinformatics Analysis of F-BOX protein of Oryza sativa
Abstract: F-BOX protein is a kind protein of containing F-box domain, it an important component of SCF complex composed of ubiquitin ligase, this kind of protein have specificity in the target protein recognition.It plays an important role in the cell cycle, signal transduction, apoptosis, development, and other physical process. in the paper, we predicted series of information such as sequence characteristics, physical and chemical properties, the transmembrane domain structure, subcellular localization, advanced structure and functional domains and so on for three kinds of rice F-BOX protein sequences. The results showed that three F-BOX proteins have different functions, but have also some similarity, for example,they are all unstable protein, and have similar phosphorylation information, similar disorders regions, they all can form the spherical protein in the whole level. Their N end sequence does not contain a signal peptide, and belong to non-secreted protein. However, they have different structure, for example, the homologous comparison and evolutionary trees for 3th sequence showed that its similarity is lower each other, and its tertiary structure results is simpler.
Key words：Oryza sativa L; F-BOX protein; ubiquitin pathway；Bioinformatics analysis
目 录
摘 要 1
引 言 2
1 材料和方法 3
1.1 材料来源 3
1.2 生物信息学分析软件 4
2 结果与分析 4
2.1 基本理化性质分析 4
2.2蛋白质跨膜区、亚细胞定位、磷酸化分析 6
2.2.1 蛋白的磷酸化分析 6
2.2.2水稻F-BOX蛋白的亚细胞定位 6
2.2.3 水稻F-BOX蛋白的跨膜区预测 7
2.3 F-BOX蛋白蛋白紊乱区、球蛋白区预测 7
2.4 保守域预测，信号肽预测 8
2.4.1信号肽预测分析 8
2.4.2保守域预测分析 9
2.5 二级结构预测分析 10
2.6 同源蛋白的比较和进化树的构建 10
2.7 F-BOX蛋白的基因结构与定位分析 12
2.8 F-BOX蛋白的电子表达分析 13
2.9 蛋白质三级结构的预测分析 14
2.9.1 蛋白质三级结构的预测及评价 14
3 结论与讨论 14
参考文献 16
致 谢 17
一种水稻F-BOX蛋白的的生物信息学分析 引 言