人源SEPT9蛋白家族不同单体模建进化和病症关联性分析
毕业论文关键词：人；SEPT9蛋白；同源模建；进化.
The modeling, evolution and correlation analysis of different SEPT9 protein isoform in human being
Abstract: Septin proteins widely exist in most eukaryotic organisms, the genes encoding this kind of proteins in many organisms appeared larger function differentiation.Sept9 protein were shown that it plays an important roles in cell pision, intracellular material transport and infection, treatment and recovery process, tumorigenesis, nerve dysfunction, and is directly related to the process of pathogenic microorganism infection. Based on the bioinformatics methods, the paper revealed the protein sequence characteristics, evolution story, and predicted its secondary structure, tertiary structure and the active site information. The results showed that the the members of the SEPT9 protein family showed small diffrence in sequence length and amino acid composition. it was a unstable globular proteins, with no trans-membrane and signal peptide; Secondary structure is given priority to with alpha helix and random curl, the tertiary structure of sept9 protein is built, and Ramachandran map test showed that the model structure in line with the rules of stereochemistry. Modern clinical medical research shows that people sept9 changes in protein expression is considered in prostate cancer, ovarian cancer, breast cancer, colon, head and neck cancer and other diseases are closely related, is also closely related to genetic neuralgia muscular atrophy. Predict the activity of the protein loci information, analysis of the drug molecule library docking results, thus for human disease treatment caused the gene mutation of reference and guidance for the design and synthesis of work.
Keywords: Human being；Septin9 protein；Homology modeling；Evolution.
目 录
摘要 1
引言 2
1 材料和方法 3
1.1 数据来源 3
1.2人源 SEPT 9蛋白的进化分析 3
1.3人源SEPT 9蛋白同源性比较和理化性质的分析 3
1.4磷酸化位点和亚细胞定位分析 4
1.5 二级结构、三级结构预测及建模的评估 4
1.6 人源SEPT9蛋白病症关联性分析 4
2 结果与分析 4
2.1人源SEPT 9蛋白同源性比较和分类分析 4
2.2人源SEPT 9蛋白的进化分析 5
2.3人源SEPT 9蛋白理化性质分析 6
2.4磷酸化位点和亚细胞定位分析 7
2.5 二级结构预测、三级结构的预测及建模结构的评估 9
2.6 SEPT9蛋白病症关联性分析 11
3讨论 11
参考文献 13
致 谢 14
人源SEPT 9蛋白家族不同单体模建、进化和病症关联性分析
引言
Septin基因广泛分布于除植物外如真菌[1]、线虫[2]、果蝇[3，4]爪蟾[5]及哺乳动物[6]等绝大多数真核生物中，具有GTPase活性的保守骨架，与细胞分裂、细胞极化、包囊运输及包膜重构有关[7]。在人类基因家族中其由14个家族成员组成，分别被命名为Sept1-14，其中Sept9定位于基因17q25.3，其含有17个外显子，编码Sept9相关蛋白。其有多个亚型（如Sept9-v1,v2,v3,v4),且各个亚基间具有结构相似性，由位于中央位置的P-loop GTP结构域，可以变动的N端和C端结构域组成，明显与染色体分离、DNA的修复、迁移、凋亡、变异等细胞功能有关。医学研究表明，Spet9与肿瘤的发生直接相关，并且在不同的肿瘤中，其表达和功能各不相同[8]。例如在急性粒细胞白血病中，Sept9与MLL基因融合而促进肿瘤的发生，而在乳腺癌、卵巢癌中，Sept9-v1高度表达，起原癌基因的作用[9]。而在结肠癌中，Sept2-v2启动子区的CPG高度甲基化表达[10]。大量实验证明，Sept9与大量肿瘤的恶性表型存在极大关系，在肿瘤的发生中起至关重要的作用，可能是治疗肿瘤潜在的靶点。因此Sept9功能的研究正成为细胞生物学和病理学研究的新热点。