增强UV-B辐射和Pb、Cd胁迫对小麦生理生化特性的影
关键词：小麦；UV-B辐射；Pb+Cd胁迫；复合胁迫；生理指标
Effects of Enhanced UV-B Radiation and Pb, Cd Stress on Physiological and Biochemical Characteristics of Wheat
Abstract: Using ordinary wheat named zhoumai 18 as materials, the effects of enhanced UV-B radiation stress, Pb+Cd stress and compound stress under different processing time on content of chlorophyll, MDA, free proline, soluble sugar, soluble protein and peroxidase activity of wheat seedling leaves were researched in pot experiment. The results showed that the total chlorophyll content of wheat seedling leaves was reduced under three kinds of stress treatments compared with the control group, the total chlorophyll content under compound stress showed the most obvious reduction; The content of MDA and free proline was higher under three kinds of stress treatments than the control group, furthermore, the increment was positive correlated with processing time, MDA content and free proline content showed the most obvious increase under compound stress; There was no significant difference in soluble sugar content between three kinds of treatment groups and the control group; Soluble protein content under three kinds of treatment groups all showed inverted "U" shape change; Peroxidase activity showed gradual reduction trend under enhanced UV-B radiation stress and gradual increase trend under Pb+Cd stress instead, furthermore, peroxidase activity under compound stress treatment became increased and then decreased gradually with the increasing processing time. The above statement showed that lots of physiological indexes of wheat seedling leaves were subject to the impact of varying degrees under three kinds of stress treatments.
Key words: Wheat; UV-B radiation; Pb+Cd stress; Compound stress; Physiological indexes
目录
摘 要 1
引言 1
1. 材料与方法 2
1.1 实验材料 2
1.2 方法 2
1.2.1 小麦幼苗培养 3
1.2.2 铅、镉胁迫液的配制 3
1.2.3 铅、镉胁迫处理 3
1.2.4 增强UV-B辐射胁迫处理 3
1.2.5 增强UV-B辐射胁迫和铅、镉胁迫复合处理 3
1.2.6 各种生理指标的测定 4
2. 结果与分析 4
2.1 各种处理下小麦幼苗叶片中叶绿素含量的变化 4
2.2 各种处理下小麦幼苗叶片中丙二醛（MDA）含量的变化 5
2.3 各种处理下小麦幼苗叶片中游离脯氨酸含量的变化 6
2.4 各种处理下小麦幼苗叶片中可溶性蛋白含量的变化 7
2.5 各种处理下小麦幼苗叶片中可溶性糖含量的变化 8
2.6 各种处理下小麦幼苗叶片中过氧化物酶活性的变化 9