脱共生菌麦长管蚜的生命表特征研究
毕业论文关键词：麦长管蚜；Regiella insecticola；净增殖率；平均世代周期
Study on the Life Table Characteristics of Sitobion avenae Cured Regiella insecticola
Abstract： The secondary endosybionts are common in the aphids. These secondary endosybionts play an important role in the growth and development of aphids. But the study found that some secondary endosybionts also have a negative impact on the growth and development of aphids. In this paper, the effects of secondary endosybiont Regiella insecticola on the life history of Sitobion avenae were studied using the aphids cured the R. insecticola in different population densities and temperatures. The net increase rate, average generation period and intrinsic rate of increase of the R. insecticola line and cured line were measured at 25 ℃, 28 ℃ and 31 ℃ for 4 h per day. The results showed that the net increase rate and the average generation period of the cured line were significantly higher than those of the R. insecticola line at 25 ℃, 28 ℃ and 31 ℃. The average generation period, net increase rate and intrinsic rate of increase were significantly impacted by R. insecticola, genetype of aphid, temperature and population density. The infection of R. insecticola has negative effects on Sitobion avenae.
Key words: Sitobion avenae;Regiella insecticola;net breeding rate;average generation period
目 录
脱共生菌麦长管蚜的生命表特征研究 1
摘要 1
关键词 1
Abstract 1
Key words 1
1 材料与方法 2
1.1麦长管蚜的采集 2
1.1.1采集地点 2
1.1.2采集方法 2
1.2盆栽小麦的种植 2
1.3麦长管蚜的脱共生处理 2
1.3.1提取麦长管蚜的DNA 2
1.3.2 次级共生菌的PCR检测 3
1.3.3麦长管蚜基因型的测定 3
1.3.4麦长管蚜体内的Regiella insecticola去除 4
1.4麦长管蚜生命表的构建 4
1.5数据处理及分析方法 4
2 结果与分析 5
2.1 25℃下麦长管蚜的生命参数表 5
2.1.1麦长管蚜的净增殖率 5
2.1.2麦长管蚜的平均世代寿命 6
2.1.3麦长管蚜的内禀增长率 6
2.2 28℃下麦长管蚜的生命参数表 8
2.2.1麦长管蚜的净增殖率 8
2.2.2麦长管蚜的平均世代寿命 8
2.2.3 麦长管蚜的内禀增长率 10
上一篇：氟环唑对小麦赤霉病的作用机制研究