乙烯和生长素在番茄果实形成过程中互作的探究
毕业论文关键词：乙烯 ；生长素；番茄；果实发育
Study on the Interaction of Ethylene and Auxin in Tomato Fruit Development
Abstract：EIL (Ethylene-insensitive3-like) is an important transcription factor in the ethylene signaling pathway. In this study, Parthenocarpic fruit set was observed in EIL co-suppressed transgenic tomato (EIL-CS line). The concentration of endogenous auxin in the fruit of EIL- CS was significantly higher than that of EIL overexpression and wild type control plants, which indicated that inhibiting the transcription of EIL could increase the content of auxin in the ovary after flowering. In order to further understand the interaction between ethylene and auxin in the process of fruit formation, the expression pattern of EIL and synthesis of auxin in buds were studied via EIL-OX, pEIL-GUS and DR5-GUS transgenic tomatoes. The results showed that the auxin biosynthesis of EIL-OX was higher than that of wild type control, but there was no significant difference in the synthesis of auxin after flowering. Under the influence of EIL, the auxin showed different synthetic characteristics before and after flowering, indicating that the mechanism of interaction between EIL and auxin was different before and after flowering.
Key words: ethylene；auxin；tomato；fruit development
目录
摘要 1
关键词 1
Abstract 1
Key words 1
引言： 1
1 材料与方法 2
1．1 试验材料 2
1．2 EIL1-OX/DR5-GUS转基因番茄的培养 2
1．3 转基因植株表型观察及取样 2
1．4 GUS染色液的配制及染色鉴定 2
1．4．1 GUS染色液的配制 2
1．4．2 GUS染色鉴定 2
1．5 EIL1浓度的测定 3
1．5．1 样品RNA的提取 3
1．5．2 cDNA合成 3
1．5．3 PCR 3
1．5．4 数据处理 3
1．6 生长素浓度测定 3
2 结果与分析 3
2．1 EIL1在番茄果实中的表达模式 3
2．2 转基因番茄表型观察 4
2．2．1 EIL对番茄植株发育的影响 4
2．2．2 EIL对番茄果实发育的影响 5
2．3 果实内源生长素浓度测定 5
2．4 通过GUS染色检测果实形成前期（开花阶段）EIL1调控生长素合成的规律 5
2．4．1 EIL1超表达对番茄果实子房发育的影响 6
2．4．2 EIL1超表达对生长素的影响 6
3 讨论 8
3．1 EIL1与单性结实 8