用9311×普通野生稻的染色体片段置换系定位控制水稻垩白率和直链淀粉含量的
毕业论文关键词：水稻，染色体片段置换系（CSSLs），垩白率，直链淀粉含量，Western blot
Detection of QTL for chalkiness and amylose content by using chromosome segment substitution lines of 9311 /O.Rufipogon in rice
Abstract:Chalkiness and amylose content are two important factors in rice which affecting the cooking and eating quality of rice, they are effected by environment and multiple genes.Chromosomal segment substitution lines(CSSLs)，in which each line carries a single or a few chromosomal segments from a donor in the genetic background of recurrent are perfect materials for QTL identification,fine mapping and map-based cloning of QTL,variety improvement and soon.By detecting rice chalkiness and amylose content , find the mutants with high chalkiness and high / low amylose content in 9311 /O.Rufipogon CSSLs,to looking for the QTLs controling those two characters.The results showed that (1)the chalkiness rate of material harvested in 2016 was lower then last year.Persistent precipitation during the grain filling stage may reduced photosynthetic products of rice,result in the grouting getting slower ,so the amyloids and proteosomes in endosperm growth slowly,which cause the chalkness getting lower.(2)We selected 2 high amylose content materials and 1 low amylose content material during the measuring, they are Q187, Q110 and Q87 which amylose content was 20.3%, 21.1% and 2.9%, and the parant of 9311 was 14.5%.(3)Spontaneous mutation of Wx of Q87 caused it become glutinous rice, so the amylose content decreased. Protein level 偶of Wx gene enhancement in Q187 and Q110 resulted amylose content increased.
Key words:Rice;Chromosome segment substitution lines(CSSLs);Chalkines;Amylose content;Western blot
目 录
摘要 3
关键词 3
Abstract 3
Key words 4
引言 4
1.材料与方法 4
1.1 实验材料 5
1.2 田间实验与种子处理 5
1.3 性状检测 5
1.3.1垩白粒率测定 5
1.3.2 直链淀粉含量测定 5
1.4 Western blot 杂交 5
2.结果与分析 6
2.1 垩白率测定 6
2.2 直链淀粉含量测定结果与分析 6
3.讨论 8
致谢 10
参考文献： 11
利用9311/普通野生稻的染色体片段置换系定位控制水稻垩白率和直链淀粉含量的QTL
引言：水稻是世界上最主要的粮食作物之一。长期以来，随着人口增长、环境问题以及国民粮食安全问题，在水稻育种和生产工作中，提高水稻产量一直是重中之重。但是近年来，随着国民生活水平的提高，人们对稻米品质要求也在不断提高。此外，国际粮食市场的竞争十分激烈，也促使了我国水稻育种目标从片面的高产转向高产与优质并举。因此稻米品质的遗传学研究成为水稻研究中重要的一部分。实验利用以9311为背景的9311/普通野生稻的染色体片段置换系214个家系作为实验材料，将鉴定其垩白率和直链淀粉含量的两项重要稻米品质性状表型值，通过调取CSSL群体材料的分子连锁图谱，寻找控制两种性状的基因片段所在位置。