分光光度法鱼体中重金属含量的分析研究
毕业论文关键词： 分光光度法；鲫鱼；重金属；健康水平
Analysis of heavy metals in fish body
Abstract: This article makes use of the spectrophotometric method to research the content of iron and chromium,which are in gills,fish and offal of different size of crucian carp in farmers’ market. We use o-phenanthroline to react with palent iron in solution at pH of 4.6,which is generated orange-red complexes iron color reaction test （λ=510nm）.We also use diphenyl hydrazine to reacts with hexavalent chromium in alkaline solution,which is generated purple complex chrome color reaction test（λ=540nm）. The linearity rang is 0.4μg/mL~2μg/mL and 0.1μg/mL~2μg/mL. The results show that the carp 1’s body length is 12.70cm and the content of iron in the carp 1’s meat is 11.932 mg / kg, gills is 17.404 mg / kg and offal is 40.616 mg / kg. The carp 2’s body length is 13.35cm and the content of iron in the carp 2’s meat is 13.767mg / kg, gills is 18.268 mg / kg and offal is 43.665 mg / kg. The carp 3’s body length is 12.45cm. The content of chromium in carp 3’s meat is 0.212mg / kg, gills is 0.428mg / kg and offal is 1.534 mg / kg. The carp 4’s body length is 14.35cm. The content of chromium in carp 4’s meat is 0.324mg / kg, gills is 0.671mg / kg and offal is 1.803 mg / kg.Thus,we can find that with the increase of body length, the amount of iron and chromium will increase. Besides, the various parts of iron or chromium content in the fish shous the order as offal> gill> fish.
KeyWords: spectrophotometric method; crucian carp; iron and chromium; health level; heavy metal
目录
1前言 1
1.1研究鱼体中重金属含量的重要意义 1
1.2鱼体中重金属检测方法的介绍 2
1.3分光光度法的原理 5
1.4分光光度法的分类 6
2 鱼体中测定铁的实验内容 7
2.1 试剂与材料 7
2.2 实验仪器 7
2.3 实验方法 7
2.3.1 工作曲线的绘制 7
2.3.2 鱼样的测定 9
2.4 实验结果与讨论 10
2.4.1 最大吸收波长的确定 10
2.4.2显色时间的选择 11
2.4.3工作曲线的绘制 12
2.4.4 鱼样测定结果 13
3 鱼体中测定铬的实验内容 15
3.1 试剂与材料 15
3.2 实验仪器 15
3.3 实验方法 15
3.3.1 工作曲线的绘制 16
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