产硫酸软骨素类多糖细菌的鉴定与诱变
毕业论文关键词： 硫酸软骨素；枯草芽孢杆菌；紫外诱变；发酵条件优化
Identification and mutation of chondroitin sulfate polysaccharides producing bacteria
Abstract:Chondroitin sulfate is present in animals of a class of glycosaminoglycan (GAG), because of its hypolipidemic, anticoagulant, anti tumor, promote coronary circulation and prevent hardening of the arteries and other biological activity, widely used in clinical medicine, health food and cosmetics. In order to select a strain of high yield of chondroitin sulfate, to provide laboratory of Bacillus subtilis as the starting strain observed starting strain morphological characteristics, through the method of UV mutagenesis of wild-type optimization, improve the content of chondroitin sulfate. The experiment, at a distance of 20cm UV light irradiation, the 2min12s mutant UV3-9 chondroitin sulfate content was the highest. Based, the fermentation conditions of UV3-9 for the optimization, won the optimal fermentation conditions: use age for 24h seed culture medium, inoculation amount 4%
inoculation with 50ml of 250ml Erlenmeyer flasks and in speed is 200rpm temperature temperature of 37 ℃shake culture box, cultivated for 48h.
Key Words: chondroitin sulfate；Bacillus subtilis；UV mutation；Optimization of fermentation conditions
目 录
1 前言 1
1.1 硫酸软骨素概述 1
1.1.1 硫酸软骨素 1
1.1.2 硫酸软骨素的药理及保健作用 2
1.1.3 国内外研究现状概述 2
1.2 枯草芽孢杆菌概述 3
1.2.1 枯草芽孢杆菌 3
1.2.2 枯草芽孢杆菌作用机理 4
1.2.3 枯草芽孢杆菌的应用 4
1.3 细菌诱变育种 4
1.4 枯草芽孢杆菌发酵条件研究 6
1.4.1 培养基对发酵的影响 6
1.4.2 其他发酵参数对发酵的影响 7
1.5 本课题研究的意义及内容 8
2 材料与方法 10
2.1 材料与仪器设备 10
2.1.1 菌种 10
2.1.2 实验材料 10
2.1.3 主要仪器和设备 10
2.1.4 实验使用器皿 11
2.1.5 培养基及试剂的制备 13
2. 2 实验方法 13
2.2.1 枯草芽孢杆菌形态观察 13
2.2.2 出发菌株生长曲线的绘制 14
2.2.3 细菌的紫外诱变 15
2.2.4 标准曲线的绘制 16
2.2.5 多糖含量测定方法 17
2.2.6 发酵条件的优化 17
3 结果与分析 18
3.1 枯草芽孢杆菌形态观察 18
3.2 枯草芽孢杆菌生长曲线的测定 19