抗植物病原真菌放线菌的筛选
毕业论文关键词：植物病原菌；放线菌；活性—化学集成筛选；次级代谢产物；生物防治
Screening of actinomycetes against plant pathogenic fungi
Abstract: Plant pathogenic fungi seriously affected the agricultural production. In order to get high value of biocontrol strains, Some actinomycetes from Jatropha of Sichuan Panzhihua and soil of Xinjiang were screened again Ustilaginoidea virens(Cooke) Takah, Fusarium solani (Mart.) Sacc, Botryosphaeria berengeriana de Not. f. sp. Parabola Nise Koganezawa et Sakuma, Rhizoctonia solani Kühn, Fusarium oxysporum Schl. f. sp. niveum (E.F.Smith) Snyder et Hansen and Fusarium moniliforme Sheld. In this paper, 13 kinds of actinomycetes were activated by crossing line method. 7 kinds of actinomycetes with antifungal activity were screened out using plate confrontation method The 7 strains were Z01, Z04, Z06, Z09, Z15, Z18, Z23, respectively. The activity of Z01, Z04, and Z18 were significantly stronger. Their fermentation broth were double-checked by the Oxford cup method. The result showed that the fermentation liquid of Z18 and Z04 had inhibitory effect on the partial test fungi. Finally, the chemical constituents of the fermentation broth of Z04 were extractd using ethyl acetate. The ethyl acetate extracts were analyzed by spectrum technology. There were 32 peaks belonging to aromatic hydrocarbons, phenols, esters, fatty acids and nitrogen-containing compounds. This study provided some experimental data for the next step in the development of biological pesticides.
Key words: Plant pathogens; Actinomycetes; Activity-chemical integrated screening; Secondary metabolites; Biological control
目录
摘 要 Ⅰ
Abstract Ⅰ
引言 1
1材料与方法 2
1.1材料 2
1.1.1放线菌菌株 2
1.1.2植物病原真菌 2
1.1.3实验仪器 3
1.2方法 3
1.2.1常用培养基的配制 3
1.2.2放线菌的纯化、保种 4
1.2.3抗植物病原真菌的活性菌株初筛 4
1.2.4抗植物病原真菌的发酵液复筛 4
1.2.5发酵液的化学筛选 5
2结果与分析 5
2.1放线菌的活化结果 5
2.2 平板对峙法初筛结果与分析 6
2.3发酵液抑菌复筛结果与分析 9