木质素酶产生真菌的分离纯化及鉴定
毕业论文关键字：木质素; 漆酶; 木质素过氧化物酶; 愈创木酚; 苯胺蓝
Separation Purification and Identification of Lignin Enzyme Producing Fungi
Abstract: As a potentially renewable resource, cellulose and lignin have great value. But the cellulose is easy to combine with hemicellulose, lignin and other matters which are hard to break down, so it must be released from lignocellulose and hemicellulose in the parcel before cellulose was used. And hemicellulose can be degraded easily, therefore, the key of cellulose degradation is the degradation of lignin, so the use of microbial degradation of lignin and cellulose has been one of the attention. The objective of this experiment is screening strains which can produce lignin enzyme. In order to separate and purify lignin-degradation fungi, fungi were screened from the rotten wood and the barks of willow, pine and grape trees. The activity of laccase and lignin peroxidase were determined to screen strains which can produce high yield laccase and lignin peroxidase. 36 strains was purified from the rotten wood and barks, with the purpose of screening high efficient lignin-degradation fungi, these purified strains were inoculated in the PDA-guaiacol plate and PDA-aniline blue plate to observe the color change, screening laccase producing fungi according to the size and depth of color ring and screening lignin peroxidase producing fungi according to the size of fade circle. 11 strains were screened and then inoculated in the liquid culture medium with static culture to determine the lignin enzyme activity, results showed that L7 was the most excellent strain with the higher lignin-degrading enzymes activities for its lignin peroxidase were up to 207.1U/L. In the liquid culture medium, the activity of laccase is smaller, and the numerical value is influenced bigger by error, so using the solid medium measure laccase activity, results showed that 1-1 was the most excellent strain with the higher laccase enzymes activities for its laccase activity were up to 1033.1U/g.
Key words: lignin; laccase; lignin peroxidase; guaiacol; aniline blue
目录
引言 1
1实验材料与方法 3
1.1样本来源 3