酵母菌发酵生产γ-癸内酯的乳化体系构建及其对产物转化的影响
毕业论文关键词： γ-癸内酯；乳化体系；蓖麻油酸
The construction of the emulsifying system of producing γ- decalactone by fermentation from yeast and its impact on the transformation of product
Abstract：γ-decalactone (GDL) is a fragrance substance lactones, mainly used for the preparation of flavor of cream, peach, citrus and coconut, etc. In this paper, the construction of the emulsifying system of producing γ-decalactone by fermentation from yeast and its impact on the transformation of product were investigated. This fermentation system is O/W, the oil phase substrate is difficult to enter the yeast, it block the utilization and conversion of substrate ricinoleic acid by yeast, so the relationship between different size of substrate and the productivity of γ-decalactone in different situations, the relationship between the size of substrate and utilization of substrate by yeast, and the relationship between the size of substrate and the productivity of γ-decalactone were investigated by adding different surfactants and cosurfactant to the medium, and compounding the surfactants to emulsify the fermentation liquor in this report. The result shows: The best effect on emulsifying and the productivity of γ-decalactone is using Tween 20 alone, and when it is added with the substrate: Tween 20=10:1, cosurfactant glycerol: Tween 20=1:2, the transformation of product γ-decalactone is increased obviously, from 0.34g/L to 0.53g/L, production of γ-decalactone increased by 55.9%. And there has some relative between the transformation of γ-decalactone and substrate size and coefficient of dispersion. In other words, there has some relative between the transformation of γ-decalactone and yeast fermentation production of emulsifying γ-decalactone emulsion system.
KeyWords： γ- decalactone；emulsifying system；ricinoleic acid
目 录
1绪论 1
1.1 本课题在国内外的发展概况及存在的问题 1
1.1.1关于发酵生产γ-癸内酯 1
1.1.2关于乳化体系的构建 2
1.2 本课题的研究目的 4
1.3 本课题的研究意义 4
1.4 本课题的研究内容 5
2实验材料和方法 5
2.1 实验材料 5
2.1.1 试剂 5
2.1.2 试验用菌种 5
2.1.3 发酵培养基 5
2.1.4 仪器设备 5
2.2 实验方法 6
2.2.1 GDL的气相检测方法及标准曲线的制作 6