水稻剑叶角度对GA3敏感性的SSR关联分析
毕业论文关键词：水稻；GA3敏感性；SSR；剑叶角度；关联分析
Mining elite allele of flag leaf angle sensitivity to exogenous GA3 in rice (Oryza sativa L.)
Abstract:It is important to explore the excellent allele of GA3 high sensitivity of rice flag leaf angle to realize the whole mechanization of rice seedling. The genetic persity, population structure and linkage disequilibrium of the natural population were analyzed by using 262 pairs of SSR markers. The mixed linear model of TASSEL software was used to analyze the sensitivity of rice flag leaf angle to GA3 susceptibility index And genome-wide association analysis with SSR markers. The results showed that four SSR loci were associated with the flag leaf angle of GA3 (P <0.01), which were RM218, RM4835, RM269 and RM25, respectively. These markers are located on rice chromosomes 3, 4, 8 and 10, respectively. And a number of carrier varieties with good allelic variation and large effect value were excavated. Carrying the RM218-155bp band of the cultivated land 18 to increase the angle of the effect of the largest leaf, the largest 13.71. Followed by carry the RM269-180bp strip of Ken Ken 18, 9.64. These favorable alleles and their vector species can be used as parents of genetic modification of rice crossbreeding.
Key words: Rice; sensitivity to GA3 ; SSR ; flag leaf angle ; Association analysis
目 录
摘要3
关键词3
Abstract3
Key words3
引言3
1 材料与方法4
1.1 供试材料 4
1.2 田间种植和试验方法4
1.3 表型鉴定 4
1.4基因组DNA提取、SSR标记基因型鉴定和等位基因鉴定 4
1.5数据分析 5
1.5.1表型分析 5
1.5.2群体遗传多样性分析5
1.5.3群体结构分析5
1.5.4 连锁不平衡分析5
1.5.5农艺性状关联位点以及优异等位变异的确定 5
2 结果与分析5
2.1 表型变异较为丰富 5
2.2遗传多样性丰富 6
2.3群体由7个亚群组成 6
2.4 7个亚群均存在显著的LD 8
2.5 与GA3处理下水稻剑叶角度相关联的SSR位点 10
2.6 GA3处理下水稻剑叶角度的优异等位变异及其典型载体材料 10
3讨论 10
致谢11
参考文献11
水稻剑叶角度对GA3敏感反应的关联分析
引言
水稻是世界上最重要的粮食作物之一，世界1/3以上的人都依靠着它所生存。但由于杂交稻的制种过程复杂，使得制种成本较高。为了增大剑叶角度,达到穗粒正常外露，提高制种产量，施用赤霉素已成为杂交稻制种过程中一个不可缺少的重要环节[1]。我国对该一项的投资就高达3 000万～5 000万元，大量的使用赤霉素不仅会加重稻粒黑粉病的发生，还会降低种子质量[2-6]，污染环境。
由于上述问题，发掘出携有剑叶角度对GA3高敏感性的优异等位变异的载体品种显得尤为重要。因此，本实验室对水稻剑叶角度对GA3敏感性指数与SSR标记进行全基因组关联分析 [7-16]，选择出有利等位变异及其载体品种可用作水稻异交性状遗传改良的亲本。