GPR40家族具有多样性对4个羊品种亲缘关系的研究
毕业论文关键词：海门羊 波尔多羊 徐淮羊 湖羊 基因 亲缘关系
GPR40 has a persity of family based on four sheep genetic relationships
Abstract: Chinese sheep breeds rich, good quality meat, but on kinship and origin of sheep breeders have been concerns, this study uses a PCR amplification, DNA sequencing and phylogenetic approach to Haimen sheep , Boer sheep , Xuhuai sheep and hu sheep four sheep breeds and genetic persity and relationship GPR40 family genes were studied to detect four sheep breeds more than 12 adult inpiduals of GPR40, expand GPR41, GPR42 and GPR43 genes sequence polymorphisms and gene sequences obtained by ClustalX (1.83) software to compare, look for polymorphic sequence, then MEGA4 software to open after comparing the nucleotide sequence, and produced a molecular phylogenetic tree Discovery 4 the presence of sheep breeds gene GPR40 family genetic persity, reveal genetic relationships among species, relevant molecular genetics information obtained for further protection, development and utilization of sheep germplasm, the establishment of a database and improve sheep production performance provides a very important scientific basis. The results show: the phylogenetic tree is pided into two branches, both in origin visible far away, Capra (Capra) wild goats (C.aegagrus) is the most likely domesticated goat wild ancestors, Texel sheep are exotic species , and compared to other sheep origin remains to be studied, the findings may provide a basis for future study.
Keywords: Haimen sheep；Boer sheep；Xu Huai sheep；Hu sheep；gene；Kinship
目录
引言 4
1 材料与方法 5
1.1 实验材料 5
1.1.1 实验动物 5
1.1.2实验试剂 5
1.2 仪器设备 6
1.3 试验方法 7
1.3.1 血液样本的采集 7
1.3.2 羊血样中基因组DNA的提取方法 7
1.3.3 电泳检测DNA的质量、计算浓度和纯化 7
1.3.4 DNA的分光光度法检测 7
1.3.5 DNA的纯化 7
1.4引物设计 8
1.4.1 扩增核糖体基因所用引物 8
1.4.2 扩增GPR40家族基因所用引物 8
1.5 统计分析方法与分子系统发生树的构建 8
2 GPR40家族基因实验结果及分析 9
2.1 4个品种GPR40家族基因序列扩增及测序 9
2.2平均碱基 13
2.3平均遗传距离 13
2.4系统进化树 14
2.5多态性分析 15