基于SSU rRNA基因序列多环芳烃污染底泥细菌多样性研究
毕业论文关键词：土壤宏基因组学 土壤多环芳烃污染 BetaProteobacteria 生物降解
Analysis based by SSU rRNA gene sequence of bacterial persity in PAHs-contaminated bottom mud
Abstract: The treatment of polycyclic aromatic hydrocarbons (PAHs) pollution, in terms of biodegrading PAHs, probably today’s most important biotechnological process. The knowledge of bacterial persity in PAHs-contaminated bottom mud could perform great value in treatment of PAHs polluted soil. In this article, we found the most dominant phyla are BetaProteobacteria in PAHs-contaminated bottom mud with metagenomic methods. We got 25710 SSU rRNA gene reads from our sample, 41.91% of which were Proteobacteria with SSU rRNA gene sequence analysis, including AlphaProteobacteria(3.14%), BetaProteobacteria(16.73%), DeltaProteobacteria(8.80%), EpsilonProteobacteria(2.28%) and GammaProteobacteria(10.72%). We also found that the taxonomy based by 16s rRNA gene sequence of this PAHs-contaminated soil microorganisms showed us that about 41.95%, 14.57% and 12.84% were respectively affiliated to class Betaproteobacteria, Gammaproteobracteria, Deltaproteobracteria. This study indicated that SSU rRNA gene sequence analysis was a very important molecular biological method to rapidly screen and identify many different strains in soil, and also a significant tool for classification. Consequently, the metagenomic data showed us the BetaProteobacteria group could be a potential polycyclic aromatic hydrocarbons (PAHs)-degrading bacteria to treat the soil of a multiple contamination by PAHs in the future. It also could be applied in enhancing degradative capacity of bioreactor for treating contaminated soil。
Keywords: Metagenome Polycyclic aromatic hydrocarbons (PAHs)
BetaProteobacteria Biodegradation
目录
引言： 4
1 材料与方法： 5
1.1 宏基因组分析 5
1.1.1 底泥样品采集 5
1.1.2 DNA的提取 5
1.1.3 Illumina sequence analysis 5
1.2 基于SSU rRNA基因序列的物种分类 6
1.3 基于16s rRNA基因序列的物种分类 6
2 结果与分析 6
3结论 10
致谢 11
参考文献 11
引言：
多环芳烃是疏水芳香族化合物,由两个或两个以上的苯环或五环以线性排列，角状排列或者稠合多苯结构组成[4]。它们是普遍存在的环境污染物[12]。它们总是以复杂的混合物形式存在于多环芳烃污染的土壤中,许多从多环芳烃污染的土壤中分离的微生物已经被发现可以生长在多环芳烃中，并将其作为唯一碳源[1]。虽然多环芳烃可能经历化学氧化、光分解、吸附和挥发,但在大多数情况下,多环芳烃的微生物降解是土壤中多环芳烃的主要降解方式[18,23]。尽管各种分子技术可以用来鉴定土壤中微生物群落,但我们对土壤中多环芳烃污染治理的知识仍然是有限的[6]。
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