亚锰对鲫鱼肾脏SOD、GST及CAT的影响
毕业论文关键词：鲫鱼肾脏、锰、超氧化物歧化酶（SOD）、谷胱甘肽S-转移酶（GST）、过氧化氢酶（CAT）。
Effect of Manganese on the activities of SOD、GST and CAT in Carassius auratus kidney
Abstract: Kidney is an important organ to all living. Its major fuction is to create urine to clear the waste of metabolism and toxic. It also can help body to achieve electrolyte and acid-base balance. Antioxidant enzyme system plays a role to clean free radical and protect body tissues. In this system, SOD, GST and CAT are called protective enzyme system. Our experiment focuses on the effective of manganese on Carassius auratus's behavior, weight, and the activities of SOD, GST and CAT. This exeperiment uses the Carassius auratus models which were exposed to 0.1 mol/L Mn、0.5 mol/L Mn、1 mol/L Mn concentrations. The activities of SOD, GST and CAT in kidney of Carassius auratus. The behavior was observed in the 96 hours. The result shows that, crucian carp will move slowly and lose weight after the exposure of Mn. The activity of SOD in fish's kidney decreased. However, GST increased after the exposure of Mn. The activity of CAT is inhibited in low Mn concentrations, but increased in high Mn concentrations.
Keywords: Carassius auratus kidney、 Manganese、Superoxide Dismutase (SOD)、Glutathione peroxidase (GST)、Catalase (CAT).
目录
引言 1
1 材料与方法 2
1.1 供试材料 2
1.1.1实验动物 2
1.1.2实验试剂 2
1.1.3实验设备 3
1.2 实验预处理 4
1.2.1样本预处理 4
1.2.2 样本采集 4
1.3指标的测定 4
1.3.1超氧化物歧化酶（SOD)的测定 4
1.3.2谷胱甘肽S-转移酶(GST)的测定 5
1.3.3过氧化氢酶(CAT)的测定 5
2 结果与分析 5
2.1 鱼的行为 6
2.2 体重变化 7
2.3 超氧化物歧化酶活力 7
2.4 谷胱甘肽S-转移酶活力 8
2.5 肾脏过氧化氢酶活力 9
3 讨论 10
参考文献 11
引言
重金属具有高毒性并且对水生生态系统有严重危害性，而且它不能降解并能在生物体内累积。另外，重金属污染不仅会扰动的生态系统的完整性也会影响人类的生物链以致引起健康问题。因此,人们已经开始重视重金属的监测与防治。重金属会在鱼不同的组织和器官累积，这使得鱼类对于生物监测的意义非常重大。此外，基于研究水生金属反应可能有助于更好的理解潜在的毒理机制[1-3]。