复合菌剂对有机磷农药毒害小麦的缓解作用
关键词：复合菌剂；有机磷农药；叶绿素含量；抗氧化酶活性；MDA含量
Relieving to Organophosphorus Pesticide Injury of Wheat by Complex Bacterium
Abstract: The combined effects of organophosphorus pesticide, the different concentrations ( 106cfu/mL,107cfu/mL,108cfu/mL) and different application methods of complex bacterium on wheat were studied, respectively. The results showed that the different concentrations of complex bacterium can alleviate the injury of organophosphorus pesticide on wheat to various degree. Complex bacterium can increase remarkably the length of root, plant height, stem diameter, dry weight, wet weight, content of chlorophyll and the activities of POD, SOD and CAT. The content of MDA was significantly decreased. The complex bacterium can obviously relieve organophosphorus pesticide restraint on the height of wheat at the concentration of 107cfu/mL. The complex bacterium can increase significantly the length of root, stem diameter, content of chlorophyll and the activities of antioxidant enzymes at the concentration of 108cfu/mL. The relieving effects of complex bacterium are different among two application methods of complex bacterium.
Key words: Complex bacterium; Organophosphorus pesticide; Chlorophyll content; Activities of antioxidant enzymes; MDA content
目 录
摘 要 1
Abstract 1
引言 2
1 材料与方法 2
1.1 试验材料 2
1.2 试验设置与处理 3
1.3 仪器和设备 3
1.4 小麦生长指标的测定 3
1.4.1 小麦根长、株高、茎粗的测量 3
1.4.2 小麦干重、湿重的测定 4
1.5 小麦生理指标的测定 4
1.5.1 叶绿素含量的测定 4
1.5.2 POD活性的测定 5
1.5.3 SOD活性的测定 6
1.5.4 CAT活性的测定 7
1.5.5 MDA含量的测定 8
2 结果与分析 9
2.1 复合菌剂与有机磷农药复合处理对小麦生长指标的影响 9
2.2 复合菌剂与有机磷农药复合处理对小麦生理指标的影响 10
2.2.1 复合菌剂与有机磷农药复合处理对小麦叶绿素含量的影响 10
2.2.2 复合菌剂与有机磷农药复合处理对小麦POD活性的影响 11
2.2.3 复合菌剂与有机磷农药复合处理对小麦SOD活性的影响 12
2.2.4 复合菌剂与有机磷农药复合处理对小麦CAT活性的影响 12
2.2.5 复合菌剂与有机磷农药复合处理对小麦MDA含量的影响 13
3 讨论 14
4 结论 15
参考文献 16
致 谢 18
复合菌剂对有机磷农药毒害小麦的缓解作用 引言
小麦是我国主要的粮食作物，随着人们生活水平的提高，小麦的食用安全问题日益受到人们的重视。当今，有机磷农药广泛应用于农作物，而较高毒性的农药，也给生态环境造成不同程度的破坏，同时使小麦农药残留增加，品质下降，直接或间接地危害人们的健康[1-2]。朱丽等[3]的研究表明，一定浓度的有机磷农药能抑制小麦的幼苗生长，提高苗死率，对小麦产生一系列毒害作用。
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