Folin- Ciocalteu紫山药多酚类物质的提取与抗氧化活性测定
关键词：多酚；提取；清除自由基；抗氧化活性。
Purple yam extraction of polyphenols
and antioxidant activity determination
Abstract: Purple yam contains large amounts of polyphenols, have beneficial in the treatment of cardiovascular disease, and has anti-oxidation. In this paper, alcohol extraction and microwave-assisted extraction method from purple yam extract polyphenols, Folin-Ciocalteu method for the determination of total phenolic content of the extract, using single factor and orthogonal experiments to determine the optimum extraction process; with vitamin C, vitamin E as a control, DPPH method for the determination of polyphenol antioxidant activity. The results show that the optimum conditions for the alcohol extraction: 50% ethanol solution, solid-liquid ratio of 1:30, extraction temperature 700C, extraction time of 60 minutes. In this condition polyphenol extracts was 1.819mg / g. DPPH radical scavenging ability results showed that: scavenging ability of polyphenols better than vitamin C and vitamin E, at the same concentrations, polyphenols have strong antioxidant activity.
Key words: Polyphenols , Extract , Free radical scavenging , Antioxidant activity .
目 录
毕业设计1
1.引言4
1.1 紫山药简介.4
1.2 紫山药营养成分研究现状.4
1.3 紫山药中的多酚类物质.4
1.3.1 花青素.5
1.3.2 原花青素.6
1.3.3 多糖.6
1.4 植物成分抗氧化活性.6
1.5 微波辅助提取.7
1.6 本课题的研究目的和意义.7
2. 实验材料与方法..7
2.1 实验材料.7
2.1.1 紫山药.7
2.1.2 试剂.8
2.1.3 主要仪器.8
2.2 试验方法.8
2.2.1 提取剂的确定.8
2.2.2 标准曲线的绘制.9
2.2.3 单因素实验.9
2.2.4 正交实验.10
2.2.5 微波辅助实验.10
2.2.6 大孔树脂纯化优化提取物.11
2.2.7 抗氧化活性测定.11
2.3 数据统计分析与作图.11
3. 结果分析与讨论..11
3.1 标准曲线.12
3.2 单因素实验..12
3.2.1 乙醇浓度.12
3.2.2 料液比.13
3.2.3 时间.13
3.2.4 温度.14
3.3 正交实验.15
3.4 微波辅助提取单因素实验.16
3.4.1 时间.16
3.4.2 功率.16
3.4.3 料液比.17
3.4.4 乙醇.18
3.5 微波辅助提取正交实验.19
3.6多酚清除DPPH.能力研究20
4. 结论..20
致谢 .21
参考文献22
紫山药多酚类物质的提取与抗氧化活性测定
1、引言
1.1.紫山药简介
紫山药也称“紫人参”，又名薯蓣和长芋，属薯蓣科，以其肥大的块根或圆柱状根供食用。块根中含有蛋白质1.5%，碳水化合物14.4%，并含有多种文生素和胆碱等，营养价值极高。富含薯蓣皂(天然的DHEA),内含有各种荷尔蒙基本物质，常吃紫山药有促进内分泌荷尔蒙的合成作用。紫山药的蛋白质含量和淀粉含量都很高，所以常吃紫山药有宜于皮肤保湿，还能促进细胞的新陈代谢。是餐桌佳肴。
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