内生真菌对铁皮石斛生长的影响
毕业论文关键词：铁皮石斛 内生真菌 促生作用 开合式间歇浸没植物生物反应器
The growth-promoting influence of endophytic fungi onDendrobium catenatum
Abstract Dendrobium catenatum is a famous endangered Chinese medicinal herb. It has extremelyhigh pharmacological and economic value. Many endophytic fungi with growth-promotingeffect present in the different parts of the Dendrobium catenatum. Identifying of twoendophytic fungi(GS222,GDB254) by morphological characteristics observation and ITSgene sequence analysis.Testing their growth-promoting activity indicators and inoculatingwith Dendrobium seedlings by using unlocked temporary immersion plant bioreactor toinvestigate the growth-promoting effect of the two endophytic fungi. The results demonstratedthat this two strains belonged to Umbelopsis .They both had the ability to produce IAA,starchand cellulose hydrolysis enzyme,also have the decomposing phosphate activity.Based onobserving the proliferation and testing Chlorophyll contents after 30 days co-culture,strainGDB254 showed the positive effect.But the co-culture experiment of strain GS222 failed dueto the fungi ball did not match the structure of the plant bioreactor.
KeyWords: Dendrobium catenatum endophytic fungi growth-promotingunlocked temporary immersion plant bioreactor
目录
摘要.
Abstract..
目录...
图清单.
表清单.
1引言...
2铁皮石斛内生真菌GDB254和GS222的鉴定
2.1实验材料...
2.1.1供试菌株
2.1.2主要仪器
2.1.3主要试剂和标准品
2.1.4真菌活化培养
2.2实验方法...
2.2.1供试菌种的活化
2.2.2内生真菌的形态学方法鉴定
2.2.3内生真菌的分子学鉴定
2.2.4铁皮石斛内生真菌同源性比对及系统发育树的构建..
2.3实验结果与分析..
2.3.1供试菌种的活化
2.3.2形态学的鉴定结果
2.3.3分子生物学的鉴定结果
2.3.4同源性比对结果.
3铁皮石斛内生真菌GDB254和GS222的促生活性指标的测定..
3.1实验材料...
3.1.1供试菌株
3.1.2主要仪器与设备
3.1.3供试培养基和试剂
3.2实验方法...
3.2.1菌株解磷能力的测定
3.2.2菌株分泌植物生长激素（IAA）能力的定性和定量实验
3.2.3菌株的细胞外水解酶活性的检测
3.3实验结果与分析..
3.3.1菌株解磷能力的检测结果
3.3.2菌株分泌植物生长激素（IAA）能力的测定结果
3.3.3菌株细胞产水解酶活性的检测结果
4利用开合式间歇浸没植物生物反应器研究菌株GDB254和GS222对铁皮石斛生长的影响.
4.1实验材料...
4.1.1供试植物材料
4.1.2主要仪器与设备
4.1.3供试培养基