复合菌剂对四溴双酚A胁迫下大豆幼苗生长及抗氧化酶的影响
关键词：复合菌剂；四溴双酚A；生长；大豆；抗氧化酶系
Effects of Compound Bacteria on Seedling Growth and Antioxidant Enzymes in Soybean Under Tetrabromobisphenol A Stress
Abstract: Using the simulation lab experiment, the combined effects of 107 cfu/mL compound bacteria and varying concentrations of tetrabromobisphenol A (TBBPA) (0, 5, 50, 100, 1000 mg/kg) on physiological-biochemical characters of soybean seedling were studied, in order to explore the influence of 107 cfu/mL compound bacteria on the seedling growth and antioxidant enzyme activities in soybean under the stress of TBBPA. The results showed that TBBPA isolate significantly decreased the contents of chlorophyll and carotenoid, whereas, malondialdehyde (MDA) contents of seedling leaves increased. The low concentration of TBBPA could increase the contents of soluble sugar and the activities of the superoxide dismutase (SOD), the peroxidase (POD) and the catalase (CAT). TBBPA with higher concentration could decrease soluble sugar content and the activities of SOD, POD and CAT. The 107 cfu/mL compound bacteria alleviated the different concentrations of TBBPA toxicity in soybean plant to various degree, but alleviative effect and TBBPA concentration had no significant correlation. Compared to sole TBBPA treatment, the compound bacteria could increase the contents of chlorophyll, carotenoids, soluble sugar，antioxidant enzyme activities of SOD, POD and CAT to various degree，whereas, MDA contents of seedling leaves decreased.
Key words: Compound bacteria; Tetrabromobisphenol A; Soybean; Growth; Antioxidant Enzyme system
目 录
摘 要 1
引言 2
1 材料与方法 2
1.1 试验材料 2
1.2 复合光合菌剂的制备 3
1.3 试验设置与处理 3
1.4 仪器和设备 3
1.5 大豆生理指标的测定 4
1.6 数据处理 9
2 结果与分析 9
2.1 复合菌剂与四溴双酚A复合处理对大豆幼苗叶绿素含量的影响 9
2.2 复合菌剂与四溴双酚A复合处理对大豆幼苗类胡萝卜素含量的影响 10
2.3 复合菌剂与四溴双酚A复合处理对大豆幼苗可溶性糖含量的影响 10
2.4 复合菌剂与四溴双酚A复合处理对大豆幼苗SOD活性的影响 11
2.5 复合菌剂与四溴双酚A复合处理对大豆幼苗POD活性的影响 12
2.6 复合菌剂与四溴双酚A复合处理对大豆幼苗CAT活性的影响 13
2.7 复合菌剂与四溴双酚A复合处理对大豆幼苗MDA含量的影响 14
3 讨论 15
4 结论 16
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