空间诱变微生物其代谢产物抗肿瘤活性的研究
关键字 铜绿假单胞菌 色素 脂肽 抗肿瘤活性 鼠李糖脂8663
毕业设计说明书（论文）外文摘要
Title study on the anti-tumor activity of metabolites of microbe mutated by simulated space environment
Abstract
Pseudomonas aeruginosa is a G- bacterium that can produce abundant metabolites including rhamnolipid, pigment and lipopeptid, etc. Rhamnolipid is not only a kind of excellent surfactant, which is widely used in chemical and medical industry, but also has the capacity of anti-tumor activity; Pigments, which belong to phenoxazine, could inhibit the growth of algae and have effects on the degradation of crude oil; Lipopeptid is usually pided into lipopeptid antibiotics and lipopeptid surfactants, both of those can rise the emulsification and bacteriostasis effect. This paper focus on pigment and lipopeptid, using them as materials to investigate the anti-tumor activity of its metabolites. The results show that pigments and lipopeptid have effects on the growth of tumor cells, which may be largely inhibited when the concentration is high.
Keywords Pseudomonas aeruginosa rhamnolipid pigment lipopeptid anti-tumor activity
目 录
1 引言 1
1.1 铜绿假单胞菌 1
1.2 代谢产物 1
1.2.1 鼠李糖脂 1
1.2.2 色素 3
1.2.3 脂肽 4
1.3 结构及性能表征 5
1.4 本论文研究内容 6
2 材料与方法 7
2.1 实验材料 7
2.1.1 菌种 7
2.1.2 肿瘤细胞株 7
2.1.3 主要仪器 7
2.1.4 主要试剂 8
2.1.5 培养基 9
2.2 实验方法 9
2.2.1 种子培养方法 9
2.2.2 摇瓶发酵培养方法 10
2.2.3 发酵液处理方法 10
2.2.4 MTT实验方法 11
2.2.5 IC50实验方法 12
3 结果与讨论 13
3.1 MTT实验结果 13
3.1.1 黄色色素 13
3.1.2 绿色色素 13
3.1.3 脂肽 14
3.2 IC50浓度值 14
3.3 MIC值 15
结 论 16
展 望 18
致 谢 19
参考文献 20
1 引言