摘要：手性分子的存在的普遍性和互为手性化合物的化学性质的差异性决定了手性化合物的用途广泛。手性醇是合成20多种芳香醇胺类药物和其它手性产品的重要中间体，所以这一类手性有机物在医药，精细品化学品甚至材料化学用品等工业生产中有着非常重要的作用。与传统的化学合成方法相比，利用微生物细胞产的羰基还原酶对手性醇的拆分不仅有高选择性，而且催化反应能耗低还对环境污染小，总体来说还能降低工业生产成本。由此可知，是否能重新找到一种新菌株，可以将底物高效转化成具有药理特性的产物就成为生物法合成芳香醇胺类药物和其它手性产品的重要中间体的关键。本文对可能具有这类催化酶且能适应反应条件的菌株进行了筛选，成功找到了一株可以催化羰基还原反应的菌株，通过基因分子鉴定该菌株确定为蜡样芽孢杆菌，命名为BacilluscereusBW；进一步对可能影响该反应的理化条件进行了一系列探究，试图优化该合成反应的条件，通过其最终的e。e。值和产率情况来判断是否能获得更高的产品效益。91760

毕业论文关键词：手性芳基醇，(4-苯基)-(吡啶-2-基)甲酮，生物催化，羰基还原酶，不对称还原

Abstract：The universal presence of chiral molecules and the special chemical properties of chiral compounds make the chiral compounds importantly in many chemicals areas。 Chiral alcohols are important intermediates for the synthesis of more than 20 kinds of  aromatic alcohols and other chiral products。 Therefore, this type of chiral organic matter has a very important role in the industrial production of pharmaceuticals, fine chemicals and even materials

chemical areas。 Compared with the traditional chemical synthesis methods, microbial cells which can produce carbonyl reductase are not only highly selective for the target product,but also more environmental-friendly than the chemical synthesis method。After all,the overall process can reduce industrial production costs。 Thus, the screening of a new fungus with active carbonyl reductaseis the key to produce aromatic alcohols and other important chiral intermediate products in biocatalytic synthesis。 In this paper, fungus that could have this kind of catalytic enzyme and adapt to the reaction conditions were screened,one of them is found successfully。By the molecular identification of this fungus’ genes,it belong to bacillus cereus and is named after Bacillus cereus BW。 A series of physical and chemical factors which could affect the reaction process were explored in attempt to optimize the conditions of  the biocatalytic synthesis reaction based on the determination of the final e。e。value and yields。

Keywords: chiral aromaticalcohols, (S)-(4-Chlorophenyl)-(pyridin-2-yl)methanol, biocatalysis, carbonyl reductase, asymmetric reduction

目录

1 前言 5

1。1 手性化分子简介及用途 5

1。2 手性醇化合物介绍及其合成背景 5

1。3羰基不对称还原酶产生菌的获得 6

1。3。1自然界中筛选羰基还原酶产生菌实例 6

1。4目标产物(S)-CPMA的研究进展 6

1。4。1基本概况 6

1。4。2(S)-(4-氯苯基)(吡啶-2-基)甲醇合成技术概况 7

1。5课题研究要求 7

1。5。1课题的研究意义 7