硫酸软骨素的提取工艺研究+文献综述
关键词：硫酸软骨素；酶解；提取
Study on the extraction of chondroitin sulfate
Abstract： Chondroitin sulfate, generally present in animal cartilage, is a mix of some acidic polysaccharides. It belongs to proteoglycan glycosaminoglycans, which is connected with protein via covalent bond. Chondroitin sulfate can prevent disease of cardiovascular, improve immunity, regulate lipid metabolism, anti-aging and others. So it has been widely used in the biomedical, food and other fields. In this paper, we use pig cartilage as raw material and enzymatic hydrolysis as extraction process. Then sulfuric acid-carbazole assay was conducted to assess extraction rate. Finally, we choose the optimum extraction technology programs and best purification assay. The results showed that, by orthogonal to the rate of chondroitin sulfate extraction, hydrolysis time had greatest impact on the extraction rate, followed by enzyme concentration. The ratio of solid to liquid and extraction temperature on the results was considered to have little impact. The best extraction conditions were : hydrolysis time 26h, enzyme concentration 120U/mL, solid-liquid ratio (w / v) 1:15, extraction temperature 55 ℃. We also found that Amberlite IRA-933 anion exchange resin column had strong capability for eluting chondroitin sulfate. And sodium chloride was a good eluent with high purification rate. Finally, we got purified chondroitin sulfate as white, odorless and salty powder.
Key Words：Chondroitin sulfate; enzyme; extract
目录
1 绪论 1
1.1硫酸软骨素概述 1
1.2硫酸软骨素的作用 1
1.3 硫酸软骨素的提取工艺方法 2
1.4课题研究目的和意义 2
1.5 课题研究的内容 3
2 实验材料和方法 3
2.1 实验试剂及仪器 3
2.2 实验方法 4
2.2.1 蛋白酶活力的测定 4
2.2.3 硫酸软骨素的不同工艺条件对提取的影响 5
2.2.3 蛋白酶最优反应条件的确定 5
2.2.4 Amberlite IRA-933 型阴离子交换树脂柱纯化 5
2.2.5 旋转蒸发浓缩 6
2.3分析方法 7
2.3.1已糖醛酸法测定硫酸软骨素含量 7
3 实验结果与讨论 8
3.1 蛋白酶活力的测定 8
3.2 胰蛋白酶酶解工艺条件的确定 9
3.2.1 预实验 9
3.2.2 酶浓度对提取效果的影响 10
3.2.3 原料与提取液之比对提取效果的影响 11
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