摘    要

     叶绿体是植物进行光合作用，将太阳能转化成化学能的重要场所，因而了解植物对光的反应机理无疑对培育高产水稻品种具有重要意义。通过前期研究，我们发现DRT1是一个在叶绿体膜上富集的蛋白而且受光调控表达。为了进一步了解是否drt1基因在水稻的光反应中发挥作用起到，我们通过在水稻drt1突变体中导入DRT1基因并研究其在强光下野生型、drt1突变体和转基因材料的叶绿体完整性及其它生理反应。论文的研究结果如下:85157

     在强光照射下，野生型水稻叶片发生褪色，而drt1突变体突变体叶片并没有发生较为明显的褪色。有趣的是，转基因材料也和野生型水稻一样表现出褪色表型这个试验研究首先证明了强光会破坏野生型水稻叶片的叶绿体结构或改变叶绿体的迁移过程；其次证明DRT1基因可以保护叶绿体完整性或影响叶绿体的光逃逸进程。 这个实验证明DRT1可能参与水稻光反应。

Abstract

Chloroplast is the major place for photosynthesis。 Therefore, exploring the  the response mechanism of rice to light will help the breeder to produce high yield rice cultivars。 Our previous work revealed that the DRT1 was chloroplast membrane-bounded protein, therefore, we expected that it may involved in light response。 To explore whether DRT1 genes play a role of the light reaction in rice, we transformed the wildtype drt1 gene into drt1 mutant background, then to compare the response difference of transgenic plants with wild type and mutant under light stimulation。 Those resμlts are as follows:

Firstly, we found under strong light irradiation, the green color of ordinary leaves in wild type faded into white and the leaf color in mutant leaf is remaining green, that indicates the normal light response happened in wild type plant under strong light stimulation。 Secondly, the drt1 mutant lost the ability of light response and transgenic plant re-acquired the ability, which indicate the DRT1 is required for light response in rice。 But so far, we could not distinguish DRT1 is involved in protecting of chloroplast integrity under strong light or affecting the speed of chloroplast moving。 All in all, Drt1 is involved in  light response in rice。

毕业论文关键词：水稻；光反应；突变体；Drt1基因；叶绿体源Q于W优H尔J论K文M网WwW.youeRw.com 原文+QQ75201.,8766

Key words: rice; Light reaction; Mutant; DRT1 genes; chloroplast

目录

1。 选题背景与意义及趋势 4

2。 实验材料 4

3。 实验步骤 4

3。1  DNA提取 4

3。2   基因鉴定 5

3。3 电泳 5

4。 目的基因克隆 6

4。1 凝胶回收 6

4。2 离心法 6

5。 转化 7

5。1 扎菌 7

5。2 质粒提取 7

5。3 酶切 7

5。4 连终载 8

5。5 连接产物转化大肠杆菌来自优W尔Y论W文C网WWw.YoueRw.com 加QQ7520,18766 细胞 8

5。6 质粒提取 8