摘 要：染料废水由于其所含染料具有色度高、化学结构稳定以及难于被降解等特点，成为目前最难处理和最受关注的废水之一。偶氮染料在工业染料中所占的比例较高。目前处理染料废水的物理和化学方法具有处理成本高、处理不彻底并且容易造成二次污染等缺点。因此，筛选高效降解偶氮染料的微生物，将其应用于染料废水的处理中具有很深远的意义。本实验从印染厂的工业废水反应池的活性污泥中分离筛选出了一株偶氮染料脱色菌株AO7-1。通过形态学、生理生化特征以及16S rDNA 基因序列分析等方法对该菌株进行鉴定。此外，研究此菌株在不同时间、盐浓度、pH 缺氧好氧和温度等条件下对偶氮染料AO7-1脱色性能的影响。探究该菌株最佳脱色条件。95033

毕业论文关键词：偶氮染料，脱色，生物降解

Abstract: Industrial wastewater has become one of the most difficult and most concerned about the wastewater because of its high color, stable chemical structure and difficult to be degraded。 Azo dyes in industrial dyes accounted for a higher proportion。 At present, the physical and chemical methods for treating dyestuff wastewater have the disadvantages of high processing cost, incomplete treatment and easy to cause secondary pollution。 Therefore, it is of great significance to screen microorganisms that efficiently degrade azo dyes and apply them to dye wastewater treatment。 In this experiment, an azo dye decolorizing bacterium AO7-1 was screened from the activated sludge of the industrial wastewater reaction tank of the printing and dyeing plant。 The strain was identified by morphological, physiological and biochemical characteristics and 16S rDNA gene sequence analysis, and the effect of this strain on the decolorization of azo dyes under different carbon sources, nitrogen source, pH , anoxic, aerobic and culture temperature was studied。 The optimum decolorization conditions were explored。

Keywords: Azo dyes; Decolorizing ; Biodegradable 

目  录

前言 3

1偶氮染料处理方法 3

1。1物理法 3

1。2化学法 4

1。3生物法 4

2 材料与方法 4

2。1供试菌和试剂 4

2。2 培养基 4

2。3 实验方法 5

2。3。1 偶氮染料金橙Ⅱ脱色降解菌的富集与分离 5

2。3。2 菌落形态及菌株显微观察 5

2。3。3 菌株16S rDNA的扩增与序列分析 5

2。3。4 菌株的系统进化分析 5

2。4 菌株的降解特性 6

2。4。1降解进程 6

2。4。2 降解温度影响 6

2。4。3 pH值影响 6

2。4。4 盐浓度的影响 6

2。4。5 缺好氧的影响 6

3 结果与分析 6

3。1 偶氮染料金橙Ⅱ脱色降解菌的富集与分离 7

3。2 降解菌的形态和培养特征 7

3。3 菌株的16S rDNA扩增与序列分析 8

3。4 菌株的系统进化分析