油菜C2H2锌指蛋白的挖掘与鉴定
毕业论文关键词：油菜；C2H2型锌指蛋白；同源物；生物信息学
Mining and appraisal of rapeseed C2H2 zinc finger protein
Abstract: Canola and model plant Arabidopsis are both belonging to cruciferae plant, their coding sequence of the genome are very conservative, a lot of function and structure gene in Arabidopsis thaliana have similar or same functions in rapeseed. A large number of comparative genome research reported that Brassica napus, Arabidopsis and other cruciferae plants have many colinearity sequence in genomic, they are conservative on genome structure and gene composition, which enables people use the information and gene function Arabidopsis biological to research their homology gene information and function in rapeseed genome, to accelerate and promote genetic improvement for rapeseed crop. In the paper, a C2H2 zinc finger proteins in arabidopsis thaliana (At3g23140) as template sequence, a protein-blast was conducted in the NCBI databank to find its homologous from rapeseed protein sequence databank, then, the structure characteristics and basic information, evolutionary information of homologous et al were obtained using bioinformatics analysis method. At last, the homologous and as template sequence (At3g23140) were compared and analyzed to predict the protein senior structure and function, which indicated that the function of C2H2 zinc finger protein in Rapeseed is similar to in Arabidopsis in the growth and development. the results showed that the C2H2 zinc figure protein in Brassinca napus including 8 sequences, their length are different with a range from 135AA-175AA and with a different PI from 6.12-9.15, so they act in alkali enviorment. They were located in extracellular or cytoplasmic. Their secondary structure main include helix and random coil.
Keywords: Brassica napus L., C2H2 zinc finger protein; Homology,Bioinformatics
目 录
摘 要 1
引 言 2
1材料和方法 3
1.1 材料来源 3
1.2 生物信息学分析软件 3
2结果和分析 3
2.1 AT3G23140锌指蛋白基本信息 4
2.2 拟南芥C2H2锌指蛋白 4
2.3 油菜C2H2型锌指蛋白基本信息 4
2.4 油菜C2H2锌指蛋白基因定位信息和结构信息 5
2.5油菜C2H2锌指蛋白亚细胞定位 6
2.6 油菜锌指蛋白的保守结构域分析 6
2.7 油菜锌指蛋白的二级结构 7
2.8 油菜锌指蛋白的多序列比对与进化分析 8
3讨 论 9