猪肉色相关候选基因TFRC的鉴定与组织表达模式分析
毕业论文关键词：猪；TFRC 基因；肉色；表达；
Pork color relevant candidate genes TFRC appraisal and tissueexpression pattern analysisAbstract: many technical indexes is available for meat quality evaluation, but in the actual consumption,color is the most easy cognitive sensory indicators to consumers,its quality directly affects the consumers'desire to buy, so color is a very important meat quality index for both producers and consumers.In theprocess of color formation, myoglobin is the main protein affecting the color,whether combination ofligand, the combination of the types of ligands and iron ion valence determines the color,but the specificmolecular regulation mechanism and related pathways are still unclear.In recent years,domestic andinternational scholars do a lot to study the identification of Color traits of quantitative trait loci and thecolor traits candidate genes,but there is few key genes which can be fully proved that influence thecolor,and effective molecular marker which can be used for improving color properties is very scarce. Inthe early study, high flux transcriptome sequencing research work has been completed.Throughbioinformatics analysis and literature review,preliminary identified the key candidate gene TFRC thataffects pork color traits.On this basis,use Real - time PCR technology to detection the differences of theexpression pattern of TFRC gene in the pig bone tissue of different color and different organizations.Theresults showed that,the expression level of TFRC genes in red muscle such as soleus and psoas ishigh,however the expression level in white muscle such as biceps femoris muscle and semitendinosus islow.Besides,the expression of TFRC genes in red muscles (soleus, psoas), liver, kidney, heart tissues are inhigher quantity,while in white muscle (biceps femoris muscle,semitendinosus), spleen, lung, duodenum,fat tissues the quantity is low.The results of this study can be used to lay the theoretical foundation forcarrying out the study of the regulatory mechanism of the pig TFRC gene in forming a nude traitsin-depth,and providing a theoretical basis for the development and utilization as the candidate gene thatinfluences the quality of pork color in future.
Key words: pig；TFRC gene；fleshcolor；expression；
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