盐胁迫下蚕豆幼苗对外源抗坏血酸的生理响应
毕业论文关键词：抗坏血酸；盐胁迫；蚕豆幼苗；缓解效应
Ascorbic Acid Mitigating the Inhibition of Salt Stress to Vicia faba Seedling Growth
Abstract: Using vicia faba to do the experiment, effects of the different concentration of ascorbic acid (0、1、2、3、4、5 g•L-1) on the growth of vicia faba seedling under Salt stress was studied by hydroponic culture. The results showed as follow：under Salt stress, the content of photosynthetic pigment and the root activity were significantly decreased than those of control[1]. The contents of MDA and the autioxidant enzyme activities were distinctly increased (P<0.01). Exogenous AsA application on the leaves significantly make the contents of photosynthetic pigment decreased, while the root activity and the autioxidant enzyme activities were significantly increased. The content of malonaldehyde (produce of membrane lipid peroxidation) was significantly decreased when the vicia faba seedlings were suffered from Salt stress for 24h(P<0.01)[2]. The results showed that exogenous AsA could effectively keep cytomembrane stabilization and lower the damage from membrane lipid peroxidation, by which could mitigate the harmful effects from Salt stress on plants, and promote the growth of vicia faba, and the effect of 3g•L-1 AsA was best[3].
Key Words：Ascorbic Acid, Salt Stress, Vicia faba Seedling, Mitigative effect
目 录
摘 要 1
Abstract 1
引言 2
1材料与方法 2
1.1 实验材料 2
1.2 处理方法 3
1.3 测定项目与方法 3
1.4 数据分析 3
2结果与分析 3
2.1 AsA喷施对盐胁迫下蚕豆幼苗POD活性的影响 3
2.2 AsA喷施对盐胁迫下蚕豆幼苗CAT活性的影响 4
2.3 AsA喷施对盐胁迫下蚕豆幼苗光合色素影响 5
2.4 AsA喷施对盐胁迫下蚕豆幼苗根系活力的影响 6
2.5 AsA喷施对盐胁迫下蚕豆幼苗SOD活性的影响 6
2.6 AsA喷施对盐胁迫下蚕豆幼苗MDA含量的影响 7
2.7 AsA喷施对盐胁迫下蚕豆幼苗超氧阴离子产生速率的影响 8
3讨论 8
致谢 12
盐胁迫下蚕豆幼苗对外源抗坏血酸的生理响应 引言
蚕豆（拉丁文：Vicia faba L.）中富含淀粉、蛋白质、纤文素和矿物质，营养价值丰富含8种必需氨基酸，因此广泛作为蔬菜、粮食、饲料等，在世界范围内都是重要的食用豆之一[4]。盐胁迫对于作物栽培来说是经常遇到的一种灾害，盐胁迫会导致作物产量的降低，严重时植株将会萎蔫、死亡，探究盐胁迫下植物所受的伤害及及其作用机理，探索植物抗盐机制及其解决措施，对植物生理学研究具有重要的理论和应用价值[5]，对于提高蚕豆产量，保障蚕豆种植业的稳定健康发展具有重要现实意义。
土壤盐渍化是因为土壤底层的盐分随着植物毛管水分上升到地表之后水分蒸发，然后盐分积累在表层的土壤中所形成的，这些盐分影响植物的生长[6]。土壤盐渍化问题亟待解决，全球大部分的耕地以及近一半的灌溉地都受到土壤盐渍化影响。盐胁迫是植物生长的重要影响因子，也会严重影响作物的产量。土壤盐渍化的情况下植物生长会受到抑制，首先植物的光合作用受到阻碍，其次植物体内产生过量活性氧，导致细胞内膜损伤，甚至造成死亡[7]。总的来说，土壤盐碱化已经严重影响着蚕豆的品质和蚕豆的产量，在当前情况下通过实验找寻蚕豆种植中减缓盐胁迫的途径是至关重要的。