黄连素在团头鲂脂肪代谢调控中的作用探究
毕业论文关键词：黄连素；脂肪代谢；调控机制；团头鲂
Exploration of the Effect of berberine on regulating lipid metabolism of Megalobrama amblycephala
Abstract: Berberine has the effects of Enhancing phagocytosis of leukocytes antipyretic and anti-oxygen free radicals, it can also protect the liver. However, there are relatively few studies in fish.In order to investigate the regulating action of the berberine in Megalobrama amblycephala’s lipornetabolism, we did an experiment that feeding 320 Megalobrama amblycephala ( weighed 80.00±0.90g) with 4 different levels of the fat and the berberine(One is the normal fat diet, the other one is the normal fat diet with berberine at 50mg/kg concentration, the third one is the high fat diet, and the last one is the high fat diet with berberine at 50mg/kg concentration) for a 8-week experimental period. Then, the growth performance, the blood biochemical indexes, the cell microstructure, and the genes related to fat metabolism were determined to show the results that comparing with the group in normal diet, the group in the normal fat diet with berberine at 50mg/kg concentration has a significant increase in the specific growth rate and a significant decrease in the feed coefficient. We can also know the berberine can not only repair the liver damage caused by high fat diet effectively, but also enhance gene expression activity. Then, the result can provide the basis on berberine’s application in aquafeed.
Key words: Berberine; Lipid metabolism; Regulating mechanism; Megalobrama amblycephala
目 录
摘要2
关键词2
Abstract2
Key words2
引言3
1材料与方法3
1.1动物和试剂 3
1.2分组与建立动物模型 3
1.3实验动物处理和数据采集4
1.4实验鱼的日料成分分析4
1.5血浆生化指标的测定4
1.6检测基因表达活性4
1.7透射电子显微镜（TEM）超微结构检查5
1.8分析方法 5
2结果与分析5
2.1生长性能差异5
2.2饲料配方以及营养成分5
2.3全鱼常规营养指标6
2.4血浆中生化指标7
2.5 RNA的表达分析8
2.6显微镜下肝细胞的观察8
3 讨论8
致谢10
参考文献10
表1 生长性能差异5
表2 饲料配方以及营养成分6
表3 常规营养指标（全鱼）6
表4 血浆脂代谢因子指标7
表5 基因表达8
黄连素在团头鲂脂肪代谢调控中的作用探究
引言：
黄连素学名小檗碱，是中药黄连的提取物，具有解热、抗菌、抗炎、抗肿瘤、增强白血球吞噬作用、抗氧自由基作用和抗消化性溃疡等作用[1] ，在以往的研究中显示黄连素具有调节机体糖脂代谢的活性，保肝的作用[2]。
在水产养殖中，脂质是重要的基本营养物质，在鱼类生长发育过程中，它能为鱼类生长提供必需的脂肪酸和磷脂，对文持生物膜结构和促进脂溶性文生素的吸收也有着重要的作用[3]。但矛盾的是，当脂肪含量提高达到起促进生长作用的水平时，就容易造成多余的脂质沉积在鱼类的肝胰脏或其他脂肪组织，就会导致脂质平衡被破坏，造反而引发生长不良，成活率低和适应环境和病原体抵抗力下降等不良情况[4]，引起鱼类脂类代谢异常和紊乱。而鱼类的疾病中肝脏的病变和损伤对鱼类影响最大，肝脏是代谢器官，因此肝脏损伤病变后，机体的免疫能力和排毒能力大大下降、身体机能紊乱，导致多种并发症。肝脏的病变中，营养性脂肪肝危害最重也最普遍。