棉花MAPK级联途径中MAPKK与MAPKK家族基因蛋白互作分析
毕业论文关键词：棉花；MAPKKK基因家族；鉴定；蛋白互作
Protein Interactions Analysis of MAPKK and MAPKKK Family in Cotton MAPK Cascade Pathway
Abstract： Cotton is the world’s most important natural textile fiber and oilseed crop. Stressors severely affect growth and development and limit crop productivity and quality. Mitogen-activated protein kinase (MAPK) cascades are highly conserved signaling modules found in all eukaryotes, including fungi,plants and animals which play a important role in stress responses of plant.At present, more and more evidence indicate that hormones and adversity can activate the MAPK cascade system, which regulates the stress response plants to the outside world.Some MAPKs and MAPKKs have been isolated from cotton, but there are few studies on the MAPKKK gene family, and a complete MAPKs signaling pathway has not yet been found in cotton.In this study, 89 MAPKKK genes were predicted by bioinformatics, and in which 52 MAPKKK genes were isolated and identified. And protein interactions between MAPKK and MAPKKK gene was preliminary studied by yeast two-hybrid technique.Which provided more basis for further study in cotton MAPK cascade pathway.
Key words: Cotton；MAPKKK gene family；Identification；Protein interactions
摘要1
关键词1
Abstract1
Key words1
引言1
1 材料与方法2
1.1 材料 2
1.1.1 植物材料2
1.1.2 细菌菌株和质粒2
1.1.3 培养基2
1.2 方法 3
1.2.1 目的基因的克隆3
1.2.2 连接产物的转化与检测3
1.2.3 基因组数据3
1.2.4 棉花MAPK级联信号途径中MAPKKK基因的注释3
1.2.5 雷蒙德氏棉MAPKKK家族成员鉴定3
1.2.6 雷蒙德氏棉MAPKKK基因多序列比对、进化和亚细胞定位分析3
1.2.7 酵母双杂交载体的构建3
1.2.8 酵母双杂交3
2 结果与分析4
2.1 棉花MAPKKK基因家族成员的鉴定与生物信息学分析4
2.1.1 MAPKKK基因家族的基因组鉴定4
2.1.2 雷蒙德氏棉MAPKKK家族成员的染色体分布4
2.1.3 雷蒙德氏棉MAPKKK家族成员的聚类、结构分析4
2.1.4 雷蒙德氏棉MAPKKK基因的氨基酸序列比对6
2.2 棉花MKK与MAPKKK家族基因蛋白互作结果分析7
3 讨论 8
3．1 MAPKKK基因家族在雷蒙德氏棉中的分布特征及分类9
3.2 MAPK级联途径中MAPKK-MAPKKK之间的互作关系9
致谢10
参考文献11
图1 雷蒙德氏棉MAPKKK基因的染色体定位4
图2 雷蒙德氏棉MAPKKK成员的进化，结构和保守基序分析5
图3 棉花中MAPKKK蛋白编码的保守氨基酸序列分析Y
图4 比较分析MAPKK蛋白MAPKKK蛋白之间的相互作用Y
图5 棉花中的MAPKK-MAPKKK互作网络图Y
棉花MAPK级联途径中MAPKK与MAPKKK家族基因蛋白互作分析
引言：棉花是世界上最重要的纤文与油料作物，产量极为重要，严重的逆境胁迫影响了棉花的生长发育。所以从作物遗传育种方面研究棉花的抗逆境胁迫具有重要意义。在植物的生命周期中，不利的环境胁迫可以通过信号传导途径将信号传递至植物细胞内，从而调控基因表达对逆境胁迫作出适应性反应，减少逆境对植物的不良影响。丝裂原活化蛋白激酶（MAPK）级联途径是普遍存在于酵母、动物和植物等真核生物中的一类保守的信号转导通路，MAPK级联途径在植物中的研究成果越来越多，不断积累的成果表明MAPK级联途径及其相关基因，在植物的生长发育、激素、细胞分裂调控以及生物和非生物胁迫等过程中具有重要作用。
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