生物活性分子在不同纳米材料界面的光谱行为研究
关键词: 表面增强拉曼光谱；紫外光谱；金属纳米颗粒；多巴胺
The spectroscopic behavior study of active biomolecules on different nanomaterials interface
Abstract: Surface Enhanced Raman Scattering (SERS) spectroscopic technique has been applied to a wide range of fields, such as life science, material science, catalytic chemistry, environmental chemistry etc. However, the study of the behavior of bioactive molecules in the interface of different nanometer material through SERS spectroscopic technique based on nano particle makes use of the advantages of both SERS spectroscopic technique and the nano particle, thus expand the breadth and depth of application of the SERS spectroscopic technique in the research fields concerned with biomolecule. This paper prepares SERS substrate material that is of good stability and high sensitivity to explore the SERS activity of dopamine and sulfydryl substituendum on surfaces of different kinds of nanometer material. This paper takes dopamine and sulfydry (the replacement of dopamine) as the target molecule, studies the spectral behavior of bioactive molecule in the interface of metal nanoparticles with the help of ultraviolet spectrum and SERS spectroscopic technique. The results imply that using sulfyryl to replace dopamine generates a stronger binding force with golden and silver nanoparticle. This paper paves the way for the further study of the structure and properties of biomolecule when interacting with nanometer materials, and the binding state and mechanism of action between molecule and metal surface. In addition, this paper is of great importance in better understanding the relation between bimolecular structure and its function.
Keywords：Surface Enhanced Raman Scattering；Ultraviolet spectrum；metal nanoparicles；Dopamine
目录
1. 文献综述
1.1 拉曼光谱与表面增强拉曼散射(SERS)光谱
1.1.1 拉曼散射的基本原理及特点
1.1.2 SERS现象及机理
1.1.3 拉曼光谱及SERS光谱的应用概况
1.2 纳米材料简介
1.2.1 纳米材料的基本效应
1.2.2 SERS基底的制备状况
1.2.3 金属纳米颗粒的重要性
1.2.4 银纳米材料和金纳米材料的制备方法
1.3 紫外光谱简介
1.3.1 分子对光的选择性吸收
1.3.2 朗伯-比尔定律
1.4 多巴胺介绍
1.5 课题的目的和意义
2. 实验部分
2.1 试剂和仪器
2.1.1 试剂
2.1.2 仪器
2.2 试剂合成
2.2.1 银胶的合成
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