江苏银杏内生放线菌的分离及其活性初步研究
毕业论文关键词：银杏 内生放线 活性
Isolation of Endophytic Actinomycetes Associated with Ginkgo Biloba in Jiangsu Province and Their Bioactivities
Abstract Ginkgo biloba L. is a unique Chinese relict species, ginkgo culture is an important part of China's ecology cultural. There exists symbiosis between endophytic actinomycetes of plants and their hosts, now that endophytic actinomycetes live in botanical tissues for a long period of time, and evolve together with their hosts. Endophytic actinomycetes can produce the same or similar bioactive agents as medicinal plants, or relay on the phytochemicals produced by the plants. It is urgent to isolate and study associated endophytes from the host plants. The main purpose of this dissertation was to investigate antimicrobial and anticancer compounds from natural products of endophytes. Through sterilization the surface of roots, stems and leaves from Ginkgo biloba, isolation of endophytic actinomycetes .We got 78 actinomycetes which 49 from root, 25 from stem and 4 from leaves. 36 of them active actinomycetes were identified, 34 strains could produce starch hydrolase, 25 strains could produce cellulose hydrolysis and 24 strains showed anti-pathogenic fungi activities. Then we analyze their plant growth-promoting activities. 13 of them have nitrogen fixation potential, 17 have solubilizing potential and 13 have produced siderophore potential.
Key Word: Ginkgo biloba Endophytic actinomycetes Activity assay
目录
摘要I
AbstractII
1引言..1
1.1银杏.1
1.2放线菌.1
1.3研究意义.2
2银杏内生菌的分离..4
2.1实验材料.4
2.2实验方法.5
2.3实验结果.7
2.4小结与讨论...10
3银杏内生放线菌活性检测11
3.1实验材料...11
3.2试验方法...12
3.3实验结果...13
3.4小结与讨论...14
4内生放线菌促生活性潜在性评价15
4.1材料...15
4.2试验方法...16
4.3结果与分析...17
4.4小结与讨论...19
总结与展望20
参考文献：21
致谢23
1 引言 1.1 银杏 1.1.1 银杏概述 银杏（Ginkgo biloba L），裸子植物门银杏科银杏属，多分布在我国温带和亚热带气候区内。银杏有极长的寿命，被称作“活化石”。其果为白色，故又称“白果”。白果营养价值丰富，药食两用，经常食用可健肺克喘[1]。人们已经从银杏中提取出了黄酮类、银杏内酯、聚戊烯醇（GBP）[2]等物质，这些物质可以提高免疫力，抑制癌细胞转移辅助放化疗[3]，在临床上，可应用于治疗冠心病、心绞痛等疾病[4]。银杏已经成为近几年药用植物研究热点之一。
下一篇：生物表面活性剂产生菌的分离及鉴定